Expression of a dehydrin gene during embryo development and drought stress in ABA-deficient mutants of sunflower (Helianthus annuus L.)

被引:37
作者
Giordani, T
Natali, L
D'Ercole, A
Pugliesi, C
Fambrini, M
Vernieri, P
Vitagliano, C
Cavallini, A
机构
[1] Univ Pisa, Sez Genet, Dipartimento Biol Piante Agr, I-56124 Pisa, Italy
[2] Scuola Super Studi Univ & Perfez S Anna, Pisa, Italy
[3] Univ Pisa, Sez Orticoltura & Floricoltura, Dipartimento Biol Piante Agr, I-56100 Pisa, Italy
关键词
abscisic acid; sunflower; dehydrin; ABA mutants; drought stress; embryo development;
D O I
10.1023/A:1006194720022
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The synthesis of a particular class of proteins, the dehydrins, is a common response to drought in plants. Dehydrins are known to be synthesized by the cell in response to abscisic acid, which represents a link between environment and nuclear activity, though dehydrin genes may be expressed even constitutively. We have investigated the relationship between abscisic acid (ABA) and accumulation of a dehydrin mRNA in sunflower, in which a dehydrin cDNA (HaDhn1a) was isolated. Tn particular, we studied changes in the steady-state level of dehydrin transcripts in two mutants for ABA synthesis and accumulation: nd-1 tan albino, non-dormant and lethal mutant with a very low ABA content and no ABA accumulation in response to stress) and w-1 (a wilty mutant, with reduced ABA accumulation) during embryo and plantlet development and drought stress. Differences between genotypes were observed through embryogenesis: w-1 shows a lower content of dehydrin transcripts in the early stages compared to control plants, indicating that ABA affects dehydrin mRNA accumulation; however, dehydrin transcripts level appears independent of ABA content in late embryogenesis. Also during drought stress in w-1 adult leaves, ABA is not quantitatively related to the steady-state level of the HaDhn1a transcripts. Finally, data on nd-1 mutant show a high level of dehydrin transcripts after drought stress in plantlet cotyledons and leaflets. These results indicate the existence of two regulation pathways of HaDhn1a transcripts accumulation, an ABA-dependent and an ABA-independent one, which may have cumulative effects.
引用
收藏
页码:739 / 748
页数:10
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