A convenient method for the structural determination of glycans related to diseases

被引：1

作者：

Ishii-Karakasa, I ^{[1
]}

机构：

[1] Kitasato Univ, Sch Med, Dept Biochem, Sagamihara, Kanagawa 2288555, Japan

来源：

NEW DEVELOPMENTS IN GLYCOMEDICINE | 2001年 / 1223卷

关键词：

nondestructive analysis; H-1-NMR spectrum; oligosaccharides;

D O I：

10.1016/S0531-5131(01)00425-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A new analytical method for the structural determination of a small quantity of cell surface glycans related to diseases was developed. Oligosaccharides liberated from cell surface glycans were pyridylaminated, and pyridylamino oligosaccharides were separated and purified by high performance liquid chromatography (HPLC). The fraction presumed to be a pyridylamino tetrasaccharide on HPLC analysis was determined to be NeuAcalpha2-3Galbeta1-3 (NeuAcalpha2-6) GalNAc-PA (I-PA) by the 600-MHz H-1-NMR spectra using the following method. Since the H-1-NMR spectra of the oligosaccharides are assumed to occupy a superposition of the spectra of each component sugar, the coupling constants of each component sugar of the oligosaccharides are almost equal to those of the corresponding monosaccharides. Based on this assumption, the best chemical shifts and coupling constants are determined by the repetition of spectral simulation using the coupling constants of the corresponding monosaccharides. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：151 / 159

页数：9

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