ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse

被引：41

作者：

Demircan, Kadir ^{[1
,2
]}

Yonezawa, Tomoko ^{[2
]}

Takigawa, Tomoyuki ^{[2
]}

Topcu, Vehap ^{[3
]}

Erdogan, Serpil ^{[4
]}

Ucar, Fatma ^{[5
]}

Armutcu, Ferah ^{[6
]}

Yigitoglu, M. Ramazan ^{[6
]}

Ninomiya, Yoshifumi ^{[2
]}

Hirohata, Satoshi ^{[2
]}

机构：

[1] Turgut Ozal Univ, Sch Med, Dept Med Biol, Ankara, Turkey

[2] Okayama Univ, Dept Mol Biol & Biochem, Grad Sch Med Dent & Pharmaceut Sci, Okayama, Japan

[3] Zekai Tahir Burak Woman Hlth Training & Res Hosp, Dept Med Genet, Ankara, Turkey

[4] Ankara Numune Training & Res Hosp, Dept Biochem, Ankara, Turkey

[5] Diskapi Yildirim Beyazit Training & Res Hosp, Dept Biochem, Ankara, Turkey

[6] Turgut Ozal Univ, Sch Med, Dept Biochem, Ankara, Turkey

来源：

NEUROSCIENCE LETTERS | 2013年 / 544卷

关键词：

ADAMTS; Aggrecanase; Spinal cord injury; Astrocytes; CHONDROITIN SULFATE PROTEOGLYCAN; OUMS-27 CHONDROSARCOMA CELLS; CEREBRAL-ARTERY OCCLUSION; HUMAN INTERVERTEBRAL DISC; CENTRAL-NERVOUS-SYSTEM; IN-VIVO; MACROPHAGE ELASTASE; HUMAN CHONDROCYTES; EXPRESSION; RAT;

D O I：

10.1016/j.neulet.2013.02.064

中图分类号：

Q189 [神经科学];

学科分类号：

071006 [神经生物学];

摘要：

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases are involved in a variety of biological processes such as angiogenesis, cancer and arthritis. ADAMTSs appears to be responsible for the cleavage of proteoglycans in several tissues including brain and cartilage. Chondroitin sulfate proteoglycans (CSPGs) maintains the integrity of the brain extracellular matrix and major inhibitory contributors for glial scar and neural plasticity. The activity of aggrecanases in the central nervous system (CNS)has been reported. ADAMTSs are an enzyme degrading CSPGs in the brain. However, there is a little knowledge regarding ADAMTSs in the CNS. We investigated the expression levels of ADAMTSs mRNAs by RT-PCR after spinal cord injury in mouse. Transcripts encoding 4 of the 19 known ADAMTSs were evaluated in the mouse spinal cord following injury. ADAMTS1, -5 and -9 expression levels were found to be upregulated. No change was observed in ADAMTS4 expression. By means of immunohistochemistry, ADAMTSs were detected in the astrocytes implying its cellular source in SCI. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI. (c) 2013 Elsevier Ireland Ltd. All rights reserved.

引用

页码：25 / 30

页数：6

共 37 条

[1]

Alterations in chondroitin sulfate proteoglycan expression occur both at and far from the site of spinal contusion injury [J].