Genetic diversity and evolutionary dynamics of Ebola virus in Sierra Leone

被引：127

作者：

Tong, Yi-Gang ^{[1
]}

Shi, Wei-Feng ^{[2
]}

Liu, Di ^{[3
]}

Qian, Jun ^{[5
]}

Liang, Long ^{[1
]}

Bo, Xiao-Chen ^{[4
]}

Liu, Jun ^{[6
]}

Ren, Hong-Guang ^{[1
]}

Fan, Hang ^{[1
]}

Ni, Ming ^{[4
]}

Sun, Yang ^{[5
]}

Jin, Yuan ^{[1
]}

Teng, Yue ^{[1
]}

Li, Zhen ^{[4
]}

Kargbo, David ^{[7
]}

Dafae, Foday ^{[7
]}

Kanu, Alex ^{[8
]}

Chen, Cheng-Chao ^{[9
]}

Lan, Zhi-Heng ^{[9
]}

Jiang, Hui ^{[9
]}

Luo, Yang ^{[10
]}

Lu, Hui-Jun ^{[5
]}

Zhang, Xiao-Guang ^{[6
]}

Yang, Fan ^{[11
,12
]}

Hu, Yi ^{[1
]}

Cao, Yu-Xi ^{[6
]}

Deng, Yong-Qiang ^{[1
]}

Su, Hao-Xiang ^{[11
,12
]}

Sun, Yu ^{[1
]}

Liu, Wen-Sen ^{[5
]}

Wang, Zhuang ^{[1
]}

Wang, Cheng-Yu ^{[5
]}

Bu, Zhao-Yang ^{[5
]}

Guo, Zhen-Dong ^{[5
]}

Zhang, Liu-Bo ^{[13
]}

Nie, Wei-Min ^{[14
]}

Bai, Chang-Qing ^{[15
]}

Sun, Chun-Hua ^{[1
]}

An, Xiao-Ping ^{[1
]}

Xu, Pei-Song ^{[4
]}

Zhang, Xiang-Li-Lan ^{[1
]}

Huang, Yong ^{[1
]}

Mi, Zhi-Qiang ^{[1
]}

Yu, Dong ^{[1
]}

Yao, Hong-Wu ^{[1
]}

Feng, Yong ^{[16
]}

Xia, Zhi-Ping ^{[5
]}

Zheng, Xue-Xing ^{[5
]}

Yang, Song-Tao ^{[5
]}

Lu, Bing ^{[1
]}

机构：

[1] State Key Lab Pathogen & Biosecur, Beijing 100071, Peoples R China

[2] Taishan Med Coll, Inst Pathogen Biol, Tai An 271000, Shandong, Peoples R China

[3] Chinese Acad Sci, Inst Microbiol, Beijing 100101, Peoples R China

[4] Beijing Key Lab New Mol Diagnost Technol, Beijing 100850, Peoples R China

[5] Key Lab Jilin Prov Zoonosis Prevent & Control, Changchun 130122, Peoples R China

[6] Chinese Ctr Dis Control & Prevent, Inst Viral Dis Control & Prevent, Beijing 102206, Peoples R China

[7] Sierra Leone Minist Hlth & Sanitat, Freetown, Sierra Leone

[8] Sierra Leone China Friendship Hosp, Freetown, Sierra Leone

[9] BGI Shenzhen, Shenzhen 518083, Peoples R China

[10] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England

[11] Chinese Acad Med Sci, Beijing 100730, Peoples R China

[12] Peking Union Med Coll, Beijing 100730, Peoples R China

[13] Chinese Ctr Dis Control & Prevent, Inst Environm Hlth & Related Prod Safet, Beijing 100021, Peoples R China

[14] 302 Hosp, Beijing 100039, Peoples R China

[15] 307 Hosp, Beijing 100071, Peoples R China

[16] Natl Hlth & Family Planning Commiss, Dept Int Cooperat, Beijing 100044, Peoples R China

[17] State Key Lab Prote, Beijing 102206, Peoples R China

[18] Chinese Ctr Dis Control & Prevent, Beijing 102206, Peoples R China

来源：

NATURE | 2015年 / 524卷 / 7563期

基金：

中国国家自然科学基金;

关键词：

MOLECULAR EVOLUTION; INFERENCE;

D O I：

10.1038/nature14490

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A novel Ebola virus (EBOV) first identified in March 2014 has infected more than 25,000 people in West Africa, resulting in more than 10,000 deaths(1,2). Preliminary analyses of genome sequences of 81 EBOV collected from March to June 2014 from Guinea and Sierra Leone suggest that the 2014 EBOV originated from an independent transmission event from its natural reservoir(3) followed by sustained human-to-human infections(4). It has been reported that the EBOV genome variation might have an effect on the efficacy of sequence-based virus detection and candidate therapeutics(5,6). However, only limited viral information has been available since July 2014, when the outbreak entered a rapid growth phase(7). Here we describe 175 full-length EBOV genome sequences from five severely stricken districts in Sierra Leone from 28 September to 11 November 2014. We found that the 2014 EBOV has become more phylogenetically and genetically diverse from July to November 2014, characterized by the emergence of multiple novel lineages. The substitution rate for the 2014 EBOV was estimated to be 1.23x10(-3) substitutions per site per year (95% highest posterior density interval, 1.04x10(-3) to 1.41x10(-3) substitutions per site per year), approximating to that observed between previous EBOV outbreaks. The sharp increase in genetic diversity of the 2014 EBOV warrants extensive EBOV surveillance in Sierra Leone, Guinea and Liberia to better understand the viral evolution and transmission dynamics of the ongoing outbreak. These data will facilitate the international efforts to develop vaccines and therapeutics.

引用

页码：93 / U191

页数：11

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