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Urinary 19-norandrosterone purification by immunoaffinity chromatography: application to gas chromatography/combustion/isotope ratio mass spectrometric analysis

被引：13
作者：
Desroches, MC
Mathurin, JC
Richard, Y
Delahaut, P
de Ceaurriz, J
机构：
[1] CREPS, Lab Natl Depistage Dopage, F-92290 Chatenay Malabry, France
[2] CER, Hormonal Lab, B-6900 Marloie, Belgium
来源：
RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2002年 / 16卷 / 05期
关键词：
D O I：
10.1002/rcm.584
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The detection of exogenous 19-norandrosterone (19-NA) in urines was investigated by using gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS). 19-NA is, for the first time to our knowledge, isolated from urinary matrix by specific immunoaffinity chomatography (IAC) before analysis. The sample preparation consisted of a preliminary purification of urine by solid-phase extraction after hydrolysis by B-glucuronidase. Unconjugated 19-NA was thus isolated by IAC and directly analysed by GC/C/IRMS. Optimisation of IAC purification was achieved and the reliability of the technique for anti-doping control is discussed. Copyright (C) 2002 John Wiley Sons, Ltd.
引用
收藏
页码：370 / 374
页数：5
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