DNA gyrase can cleave short DNA fragments in the presence of quinolone drugs

被引：21

作者：

Cove, ME ^{[1
]}

Tingey, AP ^{[1
]}

Maxwell, A ^{[1
]}

机构：

[1] UNIV LEICESTER,DEPT BIOCHEM,LEICESTER LE1 7RH,LEICS,ENGLAND

来源：

NUCLEIC ACIDS RESEARCH | 1997年 / 25卷 / 14期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1093/nar/25.14.2716

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have analysed the DNA cleavage reaction of DNA gyrase using oligonucleotides annealed to a single-stranded M13 derivative containing a preferred gyrase cleavage site, We find that gyrase can cleave duplexes down to similar to 20 bp in size in the presence of the quinolone drugs ciprofloxacin and oxolinic acid, Ciprofloxacin shows a variation in its site specificity with an apparent preference for G bases adjacent to the cleavage sites, whereas oxolinic acid stimulates cleavage predominantly at the previously determined site. With either drug, cleavage will not occur within 6 bases from the end of a DNA duplex or a nick, We suggest that cleavage site specificity with short DNA duplexes is determined by drug-DNA interactions whereas with longer fragments the positioning effect of the DNA wrap around gyrase prescribes the site of cleavage.

引用

页码：2716 / 2722

页数：7

共 59 条

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