Plant-type ferredoxin-NADP(+) reductases: A basal structural framework and a multiplicity of functions

被引:136
作者
Arakaki, AK [1 ]
Ceccarelli, EA [1 ]
Carrillo, N [1 ]
机构
[1] UNIV NACL ROSARIO, FAC CIENCIAS BIOQUIM & FARMACEUT, PROMUBIE, DIV MOL BIOL, RA-2000 ROSARIO, SANTA FE, ARGENTINA
关键词
phylogenetic relationships; flavoprotein biogenesis; FAD assembly; structure-function relationships;
D O I
10.1096/fasebj.11.2.9039955
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Ferredoxin-NADP(+) (oxido)reductase (EC 1.18.1.2, FNR) is an FAD-containing enzyme that catalyzes the reversible electron transfer between NADP(H) and electron carrier proteins such as ferredoxin and flavodoxin, Isoforms of this flavoprotein are present in chloroplasts, mitochondria, and bacteria ill which they participate in a,vide variety of redox metabolic pathways, Although ferrodoxin-NADP(+) reductases have been thoroughly investigated and their properties reviewed on several occasions, considerable advances in the understanding of these flavoenzymes have occurred in the last few years, including the characterization of cDNA and genomic clones encoding FNR proteins from plants, algae, vertebrates, and bacteria, determination of the atomic structure of a plant FNR at high resolution, and the expression of functional reductases in microorganisms like Escherichia coli and Saccharomyces cerevisiae. The aim of this article is to summarize information gained through these recent developments, including the phylogenetic relationships among ferredoxin reductases and the key structural features of the plant FNR family. Other aspects such as the catalytic mechanism of FNR and the molecular events underlying biogenesis, intracellular sorting, folding, and holoenzyme assembly of this important flavoenzyme are also discussed in some detail, Ferredoxin-NADP(+) reductases display several outstanding properties that make them excellent model proteins to address broad biological questions.
引用
收藏
页码:133 / 140
页数:8
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