S-Adenosyl-L-methionine:hydroxide adenosyltransferase:: A SAM enzyme

被引：23

作者：

Deng, Hai ^{[1
]}

Botting, Catherine H. ^{[1
]}

Hamilton, John T. G. ^{[2
]}

Russell, Rupert J. M. ^{[1
]}

O'Hagan, David ^{[1
]}

机构：

[1] Univ St Andrews, Ctr Biomol Sci, St Andrews KY16 9ST, Fife, Scotland

[2] Agri Food Biosci Inst, Agr Food & Environm Sci Div, Food Chem Branch, Belfast BT9 5PX, Antrim, North Ireland

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2008年 / 47卷 / 29期

基金：

英国生物技术与生命科学研究理事会;

关键词：

archaea; enzyme catalysis; fluorinase; nucleophilic substitution; SAM enzymes;

D O I：

10.1002/anie.200800794

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Chemical Equation Presented) Not so DUF: A DUF62 enzyme from the archaeon Pyrococcus horikoshii OT3 converts S-adenosyl-L-methionine (SAM) into adenosine through the nucleophilic attack of a hydroxide ion derived from water (see picture of the active site). The highly conserved nature of Asp68, Arg75, and His127 throughout the DUF62 protein superfamily suggests the wide-spread distribution of this novel catalytic activity in microorganisms. DUF = domain of unknown function. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：5357 / 5361

页数：5

共 23 条

[1] Enzymatic fluorination in Streptomyces cattleya takes place with an inversion of configuration consistent with an SN2 reaction mechanism [J].

Cadicamo, CD ;

Courtieu, J ;

Deng, H ;

Meddour, A ;

O'Hagan, D .

CHEMBIOCHEM, 2004, 5 (05) :685-690

[2]

COHN MS, 1977, J BIOL CHEM, V252, P8212

[3] The fluorinase from Streptomyces cattleya is also a chlorinase [J].

Deng, H ;

Cobb, SL ;

McEwan, AR ;

McGlinchey, RP ;

Naismith, JH ;

O'Hagan, D ;

Robinson, DA ;

Spencer, JB .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2006, 45 (05) :759-762

[4]

DENG H, 2006, ANGEW CHEM, V118, P773

[5] Crystal structure and mechanism of a bacterial fluorinating enzyme [J].

Dong, CJ ;

Huang, FL ;

Deng, H ;

Schaffrath, C ;

Spencer, JB ;

O'Hagan, D ;

Naismith, JH .

NATURE, 2004, 427 (6974) :561-565

[6] Discovery and characterization of a marine bacterial SAM-dependent chlorinase [J].

Eustaquio, Alessandra S. ;

Pojer, Florence ;

Noel, Joseph P. ;

Moore, Bradley S. .

NATURE CHEMICAL BIOLOGY, 2008, 4 (01) :69-74

[7] S-adenosylmethionine:: nothing goes to waste [J].

Fontecave, M ;

Atta, M ;

Mulliez, E .

TRENDS IN BIOCHEMICAL SCIENCES, 2004, 29 (05) :243-249

[8]

GHOSH AK, 1999, J CHEM SOC P1, V24, P3597

[9] Pyrococcus horikoshii sp. nov., a hyperthermophilic archaeon isolated from a hydrothermal vent at the Okinawa Trough [J].

Gonzalez, JM ;

Masuchi, Y ;

Robb, FT ;

Ammerman, JW ;

Maeder, DL ;

Yanagibayashi, M ;

Tamaoka, J ;

Kato, C .

EXTREMOPHILES, 1998, 2 (02) :123-130

[10]

GRONAN DW, 1997, MICROBIOL MOL BIOL R, V61, P429

← 1 2 3 →