A highly selective G-quadruplex-based luminescent switch-on probe for the detection of gene deletion

被引：88

作者：

He, Hong-Zhang ^{[1
]}

Chan, Daniel Shiu-Hin ^{[1
]}

Leung, Chung-Hang ^{[2
,3
]}

Ma, Dik-Lung ^{[1
]}

机构：

[1] Hong Kong Baptist Univ, Dept Chem, Kowloon Tong, Hong Kong, Peoples R China

[2] Univ Macau, State Key Lab Qual Res Chinese Med, Taipa, Peoples R China

[3] Univ Macau, Inst Chinese Med Sci, Taipa, Peoples R China

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 76期

关键词：

LABEL-FREE; DNA; AMPLIFICATION; POLYMERASE; POTASSIUM; IONS;

D O I：

10.1039/c2cc32253f

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A G-quadruplex-based switch-on luminescence assay has been developed for the detection of gene deletion using a cyclometallated iridium(III) complex as a G-quadruplex-selective probe. Upon hybridization with the target DNA, the two split G-quadruplex-forming sequences assemble into a split G-quadruplex, which greatly enhances the luminescence emission of the iridium(III) probe. The assay is simple and highly selective.

引用

页码：9462 / 9464

页数：3

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