Split DNA enzyme for visual single nucleotide polymorphism typing

被引：179

作者：

Kolpashchikov, Dmitry M. ^{[1
]}

机构：

[1] Columbia Univ, Dept Med, Div Expt Therapeut, New York, NY 10032 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2008年 / 130卷 / 10期

关键词：

D O I：

10.1021/ja711192e

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A probe that changes solution color in the presence of only one of the two DNA sequences, which differ by a single nucleotide, is reported. The probe consists of two oligodeoxyribonucleotides, which form a hydrogen peroxidase-like DNA enzyme when hybridized to the abutting fragments of the complementary analyte. The active peroxidase catalyzes oxidation of colorless substrates to the colored products. The probe allows visual detection of a mutation in Alzheimer's disease related DNA.

引用

页码：2934 / 2935

页数：2

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