Structure of the O-polysaccharide from the lipopolysaccharide from Vibrio cholerae O6

被引:19
作者
Bergström, N
Nair, GB
Weintraub, A
Jansson, PE [1 ]
机构
[1] Huddinge Univ Hosp, Karolinska Inst, Analyt Unit, Clin Res Ctr,Novum, SE-14186 Huddinge, Sweden
[2] Natl Inst Cholera & Enter Dis, Kolkata 700010, W Bengal, India
[3] Huddinge Univ Hosp, Karolinska Inst, Div Clin Bacteriol, Dept Microbiol Pathol & Immunol, S-14186 Huddinge, Sweden
关键词
lipopolysaccharide; O-antigen; bacterial polysaccharide; Vibrio cholerae diarrhea;
D O I
10.1016/S0008-6215(02)00056-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The O-polysaccharide from Vibrio cholerae O6 was isolated from the LPS by mild-acid hydrolysis and has been investigated by sugar and methylation analysis and NMR spectroscopy. The polysaccharide was also depolymerized with aqueous hydrofluoric acid to give the repeating unit and multiples thereof. The O-polysaccharide had the following tetrasaccharide repeating unit. Two O-acetyl groups are present, one of them making the GlcNAc residue fully substituted and the steric crowding considerable at the branching residue. [GRAPHICS] (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:813 / 817
页数:5
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