Efficient ends-out gene targeting in Drosophila

被引：86

作者：

Huang, Juan ^{[1
]}

Zhou, Wenke ^{[1
]}

Watson, Annie M. ^{[1
]}

Jan, Yuh-Nung ^{[2
,3
]}

Hong, Yang ^{[1
]}

机构：

[1] Univ Pittsburgh, Sch Med, Dept Cell Biol & Physiol, Pittsburgh, PA 15261 USA

[2] Univ Calif San Francisco, Howard Hughes Med Inst, Dept Physiol, San Rafael, CA 94193 USA

[3] Univ Calif San Francisco, Howard Hughes Med Inst, Dept Biochem, San Rafael, CA 94193 USA

来源：

GENETICS | 2008年 / 180卷 / 01期

关键词：

D O I：

10.1534/genetics.108.090563

中图分类号：

Q3 [遗传学];

学科分类号：

071007 [遗传学]; 090102 [作物遗传育种];

摘要：

In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-positive targeting candidates.

引用

页码：703 / 707

页数：5

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