Hematogenous vertical transmission of herpes simplex virus type 1 in mice

被引:22
作者
Burgos, JS [1 ]
Ramirez, C
Guzman-Sanchez, F
Alfaro, JM
Sastre, I
Valdivieso, F
机构
[1] Univ Autonoma Madrid, Ctr Biol Mol Severo Ochoa, Lab CX340, CSIC, E-28049 Madrid, Spain
[2] Univ Autonoma Madrid, Dept Mol Biol, E-28049 Madrid, Spain
关键词
D O I
10.1128/JVI.80.6.2823-2831.2006
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Herpes simplex virus type 1 (HSV-1) is a neurotropic virus that causes severe disease and death in newborn humans but, to date, it remains unclear how neonatal infection occurs. We show here that the vertical transmission of HSV-1 in mice is mainly hematogenous and involves the colonization of the neonate central nervous system (CNS). HSV-1 DNA was mainly detected in the blood and CNS of the offspring born to latently infected mothers; no significant differences were seen between the viral DNA concentrations in the blood of these mothers and their female progeny (either neonate or adult). The administration of acyclovir during gestation reduced or eliminated both the maternal and the neonatal viral DNA in the blood. Embryo transfer was performed to ensure (as far as possible) that only vertical hematogenous infection took place. Immunohistochemical analysis detected viral proteins in the encephalon of the offspring. Immunofluorescence studies provided immunoreactive evidence of HSV-1 proteins in the neurons of the hippocampus and showed that these viruses can molecularly reactivate after hyperthermia. Neonatal HSV-1 infection therefore appears to be mainly caused by hematogenous vertical transmission, and the viruses that colonize the offspring CNS are capable of molecular reactivation after a period of latency.
引用
收藏
页码:2823 / 2831
页数:9
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