Development of a simple restriction fragment length polymorphism (RFLP) based assay for HCV genotyping and comparative analysis with genotyping and serotyping tests

被引：37

作者：

Thiers, V

Jaffredo, F

Tuveri, R

Chodan, N

Brechot, C

机构：

[1] INST PASTEUR,CBMS,HYBRIDOTEST,F-75724 PARIS 15,FRANCE

[2] CHU NECKER,INSERM,U370,F-75730 PARIS 15,FRANCE

来源：

JOURNAL OF VIROLOGICAL METHODS | 1997年 / 65卷 / 01期

关键词：

direct sequencing; HCV genotypes; HCV RNA; LiPA; PCR; 5' UTR RFLP;

D O I：

10.1016/S0166-0934(96)02162-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The distribution between the different hepatitis C virus genotypes and subtypes has potentially important clinical and epidemiological implications. With this view a simple restriction fragment length polymorphism (RFLP) based assay was developed to identify the three major genotypes, 1, 2 and 3 and distinguish subtype 1a from 1b. This RFLP test uses a combination of three restriction endonuclease digestions, BstN1, BstU1 and Sau3a, respectively. Comparison with a 5'UTR based assay (LiPA), showed a 98% concordance with the RFLP based assay. In addition, good concordance is shown between these data and those obtained with a serological assay based on NS4 peptides. (C) 1997 Elsevier Science B.V.

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收藏

页码：9 / 17

页数：9

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