Methylation-specific PCR for detection of neoplastic DNA in biopsy washings

被引：34

作者：

Goessl, C ^{[1
]}

Müller, M ^{[1
]}

Heicappell, R ^{[1
]}

Krause, H ^{[1
]}

Schostak, M ^{[1
]}

Straub, B ^{[1
]}

Miller, K ^{[1
]}

机构：

[1] Benjamin Franklin Med Sch, Dept Urol, D-12200 Berlin, Germany

来源：

JOURNAL OF PATHOLOGY | 2002年 / 196卷 / 03期

关键词：

GSTP1; prostate carcinoma; biopsy; promoter hypermethylation; PIN; molecular pathology;

D O I：

10.1002/path.1063

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Epigenetic DNA alterations such as promoter hypermethylation of glutathione S-transferase P1 (GSTP1) in prostatic adenocarcinoma frequently constitute tumour biomarkers. Neoplastic transformation was identified in washings of prostate biopsies by GSTP1 promoter hypermethylation, using methylation-specific PCR (MSP). Twenty-six patients undergoing sextant transrectal prostate biopsies for clinically suspected prostate cancer were enrolled. All ten patients diagnosed with adenocarcinoma (100%) and four of six patients with prostatic intraepithelial neoplasia (67%), but none of ten patients with benign hyperplasia (0%), exhibited GSTP1 promoter hypermethylation in at least one out of six biopsy washings. Since this approach is transferable to various cancer entities, a sensitive and specific DNA-based analysis of biopsy material seems generally feasible without impeding routine histopathological examination. Copyright (C) 2002 John Wiley Sons, Ltd.

引用

页码：331 / 334

页数：4

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