Full structure of the lipopolysaccharide of Pseudomonas aeruginosa immunotype 5

被引：18

作者：

Bystrova, OV

Lindner, B

Moll, H

Kocharova, NA

Knirel, YA

Zahringer, U

Pier, GB

机构：

[1] Russian Acad Sci, Zelinsky Inst Organ Chem, Moscow 119991, Russia

[2] Ctr Med & Biosci, Res Ctr Borstel, D-23845 Borstel, Germany

[3] Harvard Univ, Sch Med, Brigham & Womens Hosp, Channing Lab, Boston, MA 02115 USA

来源：

BIOCHEMISTRY-MOSCOW | 2004年 / 69卷 / 02期

关键词：

lipopolysaccharide; core oligosaccharide; repeating unit; O-antigen; ethanolamine diphosphate; Pseudomonas aeruginosa;

D O I：

10.1023/B:BIRY.0000018947.60328.8d

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The lipopolysaccharide (LPS) of the opportunistic human pathogen Pseudomonas aeruginosa immunotype 5 was delipidated by mild acid hydrolysis, and the products were separated by high-performance anion-exchange chromatography and analyzed by ESI MS and NMR spectroscopy. LPS species of three types were found, including those with an unsubstituted core and the core substituted with one O-polysaccharide repeating unit or with an O-polysaccharide of a variable number of repeating units. The core region is highly phosphorylated, the major species containing two monophosphate groups and one ethanolamine diphosphate group. Based on these and published data on the O-polysaccharide structure, the full structure of the LPS of P. aeruginosa immunotype 5 was established.

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页码：170 / 175

页数：6

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