Effects of IL6 and IL1β on aFGF expression and excitotoxicity in NT2N cells

被引:20
作者
Thorns, V
Walter, GF
Licastro, F
机构
[1] Hannover Med Sch, Dept Neuropathol, D-30625 Hannover, Germany
[2] Univ Bologna, Dept Expt Pathol, I-40126 Bologna, Italy
关键词
interleukin-1; beta; interleukin-6; NT2; cells; excitotoxicity;
D O I
10.1016/S0165-5728(02)00072-3
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The interleukin-1beta (IL1beta) and interleukin-6 (IL6) have pro-inflammatory and neuroprotective functions and are elevated in many diseases of the brain. Here, mechanisms and effects of IL1beta and IL6 on neuronal survival after excitatory stimulation were investigated in vitro. IL6 upregulated the expression of the neuroprotective acidic fibroblast growth factor (aFGF) and reduced the glutamate-induced cytotoxicity. IL1beta treatment amplified the excitotoxic effects after 24 h, but longer treatment with IL1beta stimulated the neuronal release of IL6 resulting in increased levels of aFGF and a decreased excitotoxicity. These data suggest that (1) IL6 exerts protective functions by upregulating the expression of aFGF and (2) the IL6/IL1beta balance in the brain may regulate neuronal survival during neuropathological processes. (C) 2002 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:22 / 29
页数:8
相关论文
共 46 条
[1]   INTERLEUKIN-1-BETA ENHANCES SURVIVAL AND INTERLEUKIN-6 PROTECTS AGAINST MPP(+) NEUROTOXICITY IN CULTURES OF FETAL-RAT DOPAMINERGIC-NEURONS [J].
AKANEYA, Y ;
TAKAHASHI, M ;
HATANAKA, H .
EXPERIMENTAL NEUROLOGY, 1995, 136 (01) :44-52
[2]   Interleukin-1 beta-induced nitric oxide production from isolated rat islets is modulated by D-glucose and 3-isobutyl-1-methyl xanthine [J].
Andersen, HU ;
Mauricio, D ;
Karlsen, AE ;
MandrupPoulsen, T ;
Nielsen, JH ;
Nerup, J .
EUROPEAN JOURNAL OF ENDOCRINOLOGY, 1996, 134 (02) :251-259
[4]   Serum interleukin-6 and interleukin-6 soluble receptor in Alzheimer's disease [J].
Angelis, P ;
Scharf, S ;
Mander, A ;
Vajda, F ;
Christophidis, N .
NEUROSCIENCE LETTERS, 1998, 244 (02) :106-108
[5]   Molecular mechanisms of actions of interleukin-6 on the brain, with special reference to serotonin and the hypothalamo-pituitary-adrenocortical axis [J].
Barkhudaryan, N ;
Dunn, AJ .
NEUROCHEMICAL RESEARCH, 1999, 24 (09) :1169-1180
[6]   INDUCTION AND REGULATION OF INTERLEUKIN-6 GENE-EXPRESSION IN RAT ASTROCYTES [J].
BENVENISTE, EN ;
SPARACIO, SM ;
NORRIS, JG ;
GRENETT, HE ;
FULLER, GM .
JOURNAL OF NEUROIMMUNOLOGY, 1990, 30 (2-3) :201-212
[7]   USE OF THE MTT ASSAY FOR RAPID-DETERMINATION OF CHEMOSENSITIVITY OF HUMAN-LEUKEMIC BLAST CELLS [J].
CAMPLING, BG ;
PYM, J ;
GALBRAITH, PR ;
COLE, SPC .
LEUKEMIA RESEARCH, 1988, 12 (10) :823-&
[8]  
Carlson NG, 1999, J IMMUNOL, V163, P3963
[9]  
Chao CC, 1996, GLIA, V16, P276, DOI 10.1002/(SICI)1098-1136(199603)16:3<276::AID-GLIA10>3.0.CO
[10]  
2-X