Intranodal vaccination with mRNA-optimized dendritic cells in metastatic melanoma patients

被引：64

作者：

Bol, Kalijn F. ^{[1
,2
]}

Figdor, Carl G. ^{[1
]}

Aarntzen, Erik H. J. G. ^{[1
,3
]}

Welzen, Marieke E. B. ^{[4
]}

van Rossum, Michelle M. ^{[5
]}

Blokx, Willeke A. M. ^{[6
]}

van de Rakt, Mandy W. M. M. ^{[1
]}

Scharenborg, Nicole M. ^{[1
]}

de Boer, Annemiek J. ^{[1
]}

Pots, Jeanette M. ^{[1
]}

Nordkamp, Michel A. M. Olde ^{[1
]}

van Oorschot, Tom G. M. ^{[1
]}

Mus, Roel D. M. ^{[3
]}

Croockewit, Sandra A. J. ^{[7
]}

Jacobs, Joannes F. M. ^{[8
]}

Schuler, Gerold ^{[9
]}

Neyns, Bart ^{[10
]}

Austyn, Jonathan M. ^{[11
]}

Punt, Cornelis J. A. ^{[12
]}

Schreibelt, Gerty ^{[1
]}

de Vries, I. Jolanda M. ^{[1
,2
]}

机构：

[1] Radboud Univ Nijmegen, Med Ctr, Dept Tumor Immunol, Radboud Inst Mol Life Sci, Nijmegen, Netherlands

[2] Radboud Univ Nijmegen, Med Ctr, Med Oncol, Nijmegen, Netherlands

[3] Radboud Univ Nijmegen, Med Ctr, Radiol & Nucl Med, Nijmegen, Netherlands

[4] Radboud Univ Nijmegen, Med Ctr, Pharm, Nijmegen, Netherlands

[5] Radboud Univ Nijmegen, Med Ctr, Dermatol, Nijmegen, Netherlands

[6] Radboud Univ Nijmegen, Med Ctr, Pathol, Nijmegen, Netherlands

[7] Radboud Univ Nijmegen, Med Ctr, Hematol, Nijmegen, Netherlands

[8] Radboud Univ Nijmegen, Med Ctr, Lab Med, Nijmegen, Netherlands

[9] Univ Hosp Erlangen, Dept Dermatol, Erlangen, Germany

[10] Vrije Univ Brussel, Dept Med Oncol, Brussels, Belgium

[11] Univ Oxford, John Radcliffe Hosp, Nuffield Dept Surg Sci, Oxford OX3 9DU, England

[12] Univ Amsterdam, Acad Med Ctr, Dept Med Oncol, NL-1105 AZ Amsterdam, Netherlands

来源：

ONCOIMMUNOLOGY | 2015年 / 4卷 / 08期

关键词：

DC vaccines; dendritic cell; immunotherapy; melanoma; mRNA electroporation; CD8(+) T-CELLS; THERAPEUTIC VACCINATION; IMMUNE-RESPONSES; TUMOR-ANTIGENS; ELECTROPORATION; IMMUNOTHERAPY; INDUCTION; CD4(+); MATURATION; EXPRESSION;

D O I：

10.1080/2162402X.2015.1019197

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 [肿瘤学];

摘要：

Autologous dendritic cell (DC) therapy is an experimental cellular immunotherapy that is safe and immunogenic in patients with advanced melanoma. In an attempt to further improve the therapeutic responses, we treated 15 patients with melanoma, with autologous monocyte-derived immature DC electroporated with mRNA encoding CD40 ligand (CD40L), CD70 and a constitutively active TLR4 (caTLR4) together with mRNA encoding a tumor-associated antigen (TAA; respectively gp100 or tyrosinase). In addition, DC were pulsed with keyhole limpet hemocyanin (KLH) that served as a control antigen. Production of this DC vaccine with high cellular viability, high expression of co-stimulatory molecules and MHC class I and II and production of IL-12p70, was feasible in all patients. A vaccination cycle consisting of three vaccinations with up to 15 x 10(6) DC per vaccination at a biweekly interval, was repeated after 6 and 12 months in the absence of disease progression. mRNA-optimized DC were injected intranodally, because of low CCR7 expression on the DC, and induced de novo immune responses against control antigen. T cell responses against tyrosinase were detected in the skin-test infiltrating lymphocytes (SKIL) of two patients. One mixed tumor response and two durable tumor stabilizations were observed among 8 patients with evaluable disease at baseline. In conclusion, autologous mRNA-optimized DC can be safely administered intranodally to patients with metastatic melanoma but showed limited immunological responses against tyrosinase and gp100.

引用

页数：11

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