Characterization of Norwalk-like virus associated with gastroenteritis in Indonesia

被引：18

作者：

Subekti, DS

Tjaniadi, P

Lesmana, M

Simanjuntak, C

Komalarini, S

Digdowirogo, H

Setiawan, B

Corwin, AL

Campbell, JR

Porter, KR

Oyofo, BA

机构：

[1] USN, Med Res Unit 2, Jakarta, Indonesia

[2] Trisakti Univ, Fac Med, Jakarta, Indonesia

[3] Minist Hlth, Natl Inst Hlth Res & Dev, Jakarta, Indonesia

[4] Sumber Waras Hosp, Jakarta, Indonesia

[5] Harapan Kita Hosp, Jakarta, Indonesia

[6] Persahabatan Hosp, Jakarta, Indonesia

来源：

JOURNAL OF MEDICAL VIROLOGY | 2002年 / 67卷 / 02期

关键词：

Norwalk-like viruses (NLVs); diarrhea; RT-PCR; sequence;

D O I：

10.1002/jmv.2215

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Norwalk Virus and Norwalk-like viruses (NLVs) are reportedly responsible for 2.5-4.0% of non-bacterial acute gastroenteritis (NBAG) worldwide. To help clarify the impact of NLVs on NBAG in Indonesia, stool specimens from 102 patients, 74 with NBAG and 28 with BAG, were screened for the presence of NLVs, using a reverse transcription-polymerase chain reaction (RT-PCR) assay. The specimens were subtyped using prototype-specific oligonucleotide probes and were sequenced and compared with published NLV sequences. Of the 102 specimens examined, 31 (30%) were found to be positive for NLVs. Type-specific probe analysis of the RT-PCR products indicated that 31 isolates hybridized to UK1 (Taunton agent) and UK3/4 (Hawaii agent/Snow Mountain agent) prototype strains. The results of this study indicate that prototype strains of NV or NLVs co-circulate in Indonesia and contribute to the overall level of acute gastroenteritis throughout the region. (C) 2002 Wiley-Liss, Inc.

引用

页码：253 / 258

页数：6