Characterization and fine localization of two new genes in Xq28 using the genomic sequence EST database screening approach

被引：15

作者：

Faranda, S

Frattini, A

Zucchi, I

Patrosso, C

Milanesi, L

Montagna, C

Vezzoni, P

机构：

[1] Ist. di Tecnol. Biomediche Avanzate, Consiglio Nazionale delle Ricerche, 20131, Milan

来源：

GENOMICS | 1996年 / 34卷 / 03期

关键词：

D O I：

10.1006/geno.1996.0293

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two new genes were identified and mapped by searching the EST databases with genomic sequences obtained from putative CpG islands of the rodent-human hybrid X3000. Previous mapping of these CpG islands in the proximity of the host cell factor (HCFC1) and GdX genes automatically localized these two new genes to Xq28 in the interval between the L1 cell adhesion molecule (L1CAM) and the glucose-6-phosphate dehydrogenase (G6PD) loci. Both genes are relatively short, contain an ORF of 261 and 105 amino acids, respectively, and are ubiquitously expressed, Combining sequencing of selected CpG islands, derived from hybrids containing small portions of the human genome, with an EST database search is an easy method of identifying and mapping new genes to specific regions of the genome. (C) 1996 Academic Press, Inc.

引用

页码：323 / 327

页数：5

共 17 条

[11] THE EXON-INTRON ORGANIZATION OF THE HUMAN X-LINKED GENE (FLN1) ENCODING ACTIN-BINDING PROTEIN-280 [J].