Two novel thio-arsenosugars in scallops identified with HPLC-ICPMS and HPLC-ESMS

被引:43
作者
Kahn, M [1 ]
Raml, R [1 ]
Schmeisser, E [1 ]
Vallant, B [1 ]
Francesconi, KA [1 ]
Goessler, W [1 ]
机构
[1] Graz Univ, Inst Chem Analyt Chem, A-8010 Graz, Austria
关键词
arsenic; bioavailability; contaminant uptake; speciation (nonmetals);
D O I
10.1071/EN05045
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Two new thio-arsenosugars were identified in aqueous and methanol extracts from the gonad and the muscle of the great scallop by using high-performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICPMS) and HPLC-electrospray ionization mass spectrometry (ESMS). The gonad of the scallops contained thio-arsenosugar-sulfonate [0.067 mg (As) kg(-1); dry mass +/- 3.7%] and thio-arsenosugar-sulfate [0.267 mg (As) kg(-1); dry mass +/- 1.2%]. The muscle also contained both thio-arsenosugar-sulfonate [0.030 mg(As)kg(-1); dry mass +/- 2.6%] and thio-arsenosugar-sulfate [0.200 mg(As)kg(-1); dry mass +/- 2.6%]. Methanol increased the extraction efficiency threefold for the two thio-arsenosugars as compared to water. Fresh and freeze-dried samples from both parts of the scallop showed no significant difference with respect to the concentration of the two thio-arsenosugars. In order to confirm the presence of the thio-arsenosugars, a new method for their determination with HPLC-ESMS was developed. Under optimized conditions [Hamilton PRP-X100; 150 by 1.0 mm; 20 mM NH4HCO3 with 55% (v/v) methanol at pH 10.3] we succeeded in determining the pseudomolecular ions of thio-arsenosugar-glycerol, thio-arsenosugar-phosphate, thio-arsenosugar-sulfonate, and thio-arseno sugar-sulfate at concentrations less than 5 mu g (As) dm(-3) in standard solutions and real samples.
引用
收藏
页码:171 / 176
页数:6
相关论文
共 13 条
[1]  
Edmonds J., 2003, ORGANOARSENIC COMPOU, P195, DOI DOI 10.1002/0470867868.CH5
[2]   Hyphenating liquid phase separation techniques with mass spectrometry: on-line or off-line [J].
Edwards, E ;
Thomas-Oates, J .
ANALYST, 2005, 130 (01) :13-17
[3]  
Francesconi KA, 1997, ADV INORG CHEM, V44, P147
[4]   ARSENIC-CONTAINING RIBOSIDES FROM THE BROWN ALGA SARGASSUM-LACERIFOLIUM - X-RAY MOLECULAR-STRUCTURE OF 2-AMINO-3-[5'-DEOXY-5'-(DIMETHYLARSINOYL)-RIBOSYLOXY]PROPANE-1-SULFONIC ACID [J].
FRANCESCONI, KA ;
EDMONDS, JS ;
STICK, RV ;
SKELTON, BW ;
WHITE, AH .
JOURNAL OF THE CHEMICAL SOCIETY-PERKIN TRANSACTIONS 1, 1991, (11) :2707-2716
[5]   Accurate quantification and transformation of arsenic compounds during wet ashing with nitric acid and microwave assisted heating [J].
Goessler, W ;
Pavkov, M .
ANALYST, 2003, 128 (06) :796-802
[6]   Arsinothioyl-sugars produced by in vitro incubation of seaweed extract with liver cytosol analysed by HPLC coupled simultaneously to ES-MS and ICP-MS [J].
Hansen, HR ;
Jaspars, M ;
Feldmann, J .
ANALYST, 2004, 129 (11) :1058-1064
[7]   2-dimethylarsinothioyl acetic acid identified in a biological sample: The first occurrence of a mammalian arsinothioyl metabolite [J].
Hansen, HR ;
Pickford, R ;
Thomas-Oates, J ;
Jaspars, M ;
Feldmann, J .
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2004, 43 (03) :337-340
[8]  
KAHN M, 2005, EUR WINT C PLASM SPE
[9]  
KANAKI K, 2005, EUR WINT C PLASM SPE
[10]   Arsenic speciation in scallops [J].
Lai, VWM ;
Cullen, WR ;
Ray, S .
MARINE CHEMISTRY, 1999, 66 (1-2) :81-89