Electrically regulated differentiation of skeletal muscle cells on ultrathin graphene-based films

被引：56

作者：

Ahadian, Samad ^{[1
]}

Ramon-Azcon, Javier ^{[1
]}

Chang, Haixin ^{[1
]}

Liang, Xiaobin ^{[1
]}

Kaji, Hirokazu ^{[2
]}

Shiku, Hitoshi ^{[3
]}

Nakajima, Ken ^{[1
]}

Ramalingam, Murugan ^{[1
,4
,5
,6
]}

Wu, Hongkai ^{[6
]}

Matsue, Tomokazu ^{[1
,3
]}

Khademhosseini, Ali ^{[1
,7
,8
,9
,10
,11
]}

机构：

[1] Tohoku Univ, WPI Adv Inst Mat Res, Sendai, Miyagi 9808577, Japan

[2] Tohoku Univ, Grad Sch Engn, Dept Bioengn & Robot, Sendai, Miyagi 9808579, Japan

[3] Tohoku Univ, Grad Sch Environm Studies, Sendai, Miyagi 9808579, Japan

[4] Inst Stem Cell Biol & Regenerat Med, Ctr Stem Cell Res, Vellore 632002, Tamil Nadu, India

[5] Univ Strasbourg, Fac Chirurg Dent, INSERM, U977, F-67085 Strasbourg, France

[6] Hong Kong Univ Sci & Technol, Dept Chem, Clear Water Bay, Hong Kong, Peoples R China

[7] Harvard Univ, Brigham & Womens Hosp, Ctr Biomed Engn, Dept Med,Sch Med, Cambridge, MA 02139 USA

[8] MIT, Harvard Mit Div Hlth Sci & Technol, Cambridge, MA 02139 USA

[9] Harvard Univ, Wyss Inst Biol Inspired Engn, Boston, MA 02115 USA

[10] Kyung Hee Univ, Sch Dent, Dept Maxillofacial Biomed Engn, Seoul 130701, South Korea

[11] Kyung Hee Univ, Sch Dent, Inst Oral Biol, Seoul 130701, South Korea

来源：

RSC ADVANCES | 2014年 / 4卷 / 19期

关键词：

OSTEOGENIC DIFFERENTIATION; MYOBLAST DIFFERENTIATION; CARBON NANOTUBES; FOCAL ADHESION; OXIDE-FILMS; STEM-CELLS; IN-VIVO; TISSUE; NANOMATERIALS; STIMULATION;

D O I：

10.1039/c3ra46218h

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

The electrical conductivity of graphene provides a unique opportunity to modify the behavior of electrically sensitive cells. Here, we demonstrate that C2C12 myoblasts that were cultured on ultrathin thermally reduced graphene (TR-Graphene) films had more favorable cell adhesion and spreading compared to those on graphene oxide (GO) and glass slide substrates, comparable with conventional Petri dish. More importantly, we demonstrate that electrical stimulation significantly enhanced myoblast cell differentiation on a TR-Graphene substrate compared to GO and glass slide surfaces as confirmed by the expression of myogenic genes and proteins. These results highlight the potential applications of graphene-based materials for cell-based studies, bioelectronics, and biorobotics.

引用

页码：9534 / 9541

页数：8

共 49 条

[1]

Electrical stimulation as a biomimicry tool for regulating muscle cell behavior [J].