共 41 条
Toward xeno-free culture of human embryonic stem cells
被引:128
作者:

Mallon, Barbara S.
论文数: 0 引用数: 0
h-index: 0
机构:
NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA

Park, Kye-Yoon
论文数: 0 引用数: 0
h-index: 0
机构:
NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA

Chen, Kevin G.
论文数: 0 引用数: 0
h-index: 0
机构:
NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA

Hamilton, Rebecca S.
论文数: 0 引用数: 0
h-index: 0
机构:
NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA

McKay, Ronald D. G.
论文数: 0 引用数: 0
h-index: 0
机构:
NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA
机构:
[1] NINDS, NIH, Stem Cell Unit, Bethesda, MD 20892 USA
关键词:
human embryonic stem cells;
MEF;
feeder-free cell culture;
matrigel;
D O I:
10.1016/j.biocel.2005.12.014
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
The culture of human embryonic stem cells (hESCs) is limited, both technically and with respect to clinical potential, by the use of mouse embryonic fibroblasts (MEFs) as a feeder layer. The concern over xenogeneic contaminants from the mouse feeder cells may restrict transplantation to humans and the variability in MEFs from batch-to-batch and laboratory-to-laboratory may contribute to some of the variability in experimental results. Finally, use of any feeder layer increases the work load and subsequently limits the large-scale culture of human ES cells. Thus, the development of feeder-free cultures will allow more reproducible culture conditions, facilitate scale-up and potentiate the clinical use of cells differentiated from hESC cultures. In this review, we describe various methods tested to culture cells in the absence of MEF feeder layers and other advances in eliminating xenogeneic products from the culture system. (c) 2006 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1063 / 1075
页数:13
相关论文
共 41 条
- [1] No evidence for infection of human embryonic stem cells by feeder cell-derived murine leukemia viruses[J]. STEM CELLS, 2005, 23 (06) : 761 - 771Amit, M论文数: 0 引用数: 0 h-index: 0机构: Hannover Med Sch, LEBAO, D-30659 Hannover, GermanyWinkler, ME论文数: 0 引用数: 0 h-index: 0机构: Hannover Med Sch, LEBAO, D-30659 Hannover, GermanyMenke, S论文数: 0 引用数: 0 h-index: 0机构: Hannover Med Sch, LEBAO, D-30659 Hannover, GermanyBrüning, E论文数: 0 引用数: 0 h-index: 0机构: Hannover Med Sch, LEBAO, D-30659 Hannover, GermanyBüscher, K论文数: 0 引用数: 0 h-index: 0机构: Hannover Med Sch, LEBAO, D-30659 Hannover, Germany论文数: 引用数: h-index:机构:Haverich, A论文数: 0 引用数: 0 h-index: 0机构: Hannover Med Sch, LEBAO, D-30659 Hannover, GermanyItskovitz-Eldor, J论文数: 0 引用数: 0 h-index: 0机构: Hannover Med Sch, LEBAO, D-30659 Hannover, GermanyMartin, U论文数: 0 引用数: 0 h-index: 0机构: Hannover Med Sch, LEBAO, D-30659 Hannover, Germany
- [2] Feeder layer- and serum-free culture of human embryonic stem cells[J]. BIOLOGY OF REPRODUCTION, 2004, 70 (03) : 837 - 845Amit, M论文数: 0 引用数: 0 h-index: 0机构: Rambam Med Ctr, Dept Obstet & Gynecol, IL-31096 Haifa, IsraelShariki, C论文数: 0 引用数: 0 h-index: 0机构: Rambam Med Ctr, Dept Obstet & Gynecol, IL-31096 Haifa, IsraelMargulets, V论文数: 0 引用数: 0 h-index: 0机构: Rambam Med Ctr, Dept Obstet & Gynecol, IL-31096 Haifa, IsraelItskovitz-Eldor, J论文数: 0 引用数: 0 h-index: 0机构: Rambam Med Ctr, Dept Obstet & Gynecol, IL-31096 Haifa, Israel
- [3] Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers[J]. STEM CELLS, 2005, 23 (04) : 489 - 495Beattie, GM论文数: 0 引用数: 0 h-index: 0机构: Univ Calif San Diego, Dept Pediat, Whittier Inst, San Diego, CA 92103 USALopez, AD论文数: 0 引用数: 0 h-index: 0机构: Univ Calif San Diego, Dept Pediat, Whittier Inst, San Diego, CA 92103 USABucay, N论文数: 0 引用数: 0 h-index: 0机构: Univ Calif San Diego, Dept Pediat, Whittier Inst, San Diego, CA 92103 USAHinton, A论文数: 0 引用数: 0 h-index: 0机构: Univ Calif San Diego, Dept Pediat, Whittier Inst, San Diego, CA 92103 USAFirpo, MT论文数: 0 引用数: 0 h-index: 0机构: Univ Calif San Diego, Dept Pediat, Whittier Inst, San Diego, CA 92103 USAKing, CC论文数: 0 引用数: 0 h-index: 0机构: Univ Calif San Diego, Dept Pediat, Whittier Inst, San Diego, CA 92103 USAHayek, A论文数: 0 引用数: 0 h-index: 0机构: Univ Calif San Diego, Dept Pediat, Whittier Inst, San Diego, CA 92103 USA
- [4] Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001[J]. STEM CELLS AND DEVELOPMENT, 2004, 13 (06) : 585 - 597Brimble, SN论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USAZeng, XM论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USAWeiler, DA论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USALuo, YQ论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USALiu, Y论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USALyons, IG论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USAFreed, WJ论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USARobins, AJ论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USARao, MS论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USASchulz, TC论文数: 0 引用数: 0 h-index: 0机构: BresaGen Inc, Athens, GA 30605 USA
- [5] Characterization and differentiation of human embryonic stem cells[J]. CLONING AND STEM CELLS, 2003, 5 (01) : 79 - 88Carpenter, MK论文数: 0 引用数: 0 h-index: 0机构: Geron Corp, Menlo Pk, CA 94025 USARosler, E论文数: 0 引用数: 0 h-index: 0机构: Geron Corp, Menlo Pk, CA 94025 USARao, MS论文数: 0 引用数: 0 h-index: 0机构: Geron Corp, Menlo Pk, CA 94025 USA
- [6] Functional expression cloning of Nanog, a pluripotency sustaining factor in embryonic stem cells[J]. CELL, 2003, 113 (05) : 643 - 655Chambers, I论文数: 0 引用数: 0 h-index: 0机构: Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, ScotlandColby, D论文数: 0 引用数: 0 h-index: 0机构: Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, ScotlandRobertson, M论文数: 0 引用数: 0 h-index: 0机构: Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, ScotlandNichols, J论文数: 0 引用数: 0 h-index: 0机构: Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, ScotlandLee, S论文数: 0 引用数: 0 h-index: 0机构: Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, ScotlandTweedie, S论文数: 0 引用数: 0 h-index: 0机构: Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, ScotlandSmith, A论文数: 0 引用数: 0 h-index: 0机构: Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland Univ Edinburgh, Inst Stem Cell Res, Edinburgh EH9 3JQ, Midlothian, Scotland
- [7] Human adult marrow cells support prolonged expansion of human embryonic stem cells in culture[J]. STEM CELLS, 2003, 21 (02) : 131 - 142Cheng, LZ论文数: 0 引用数: 0 h-index: 0机构: Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USA Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USAHammond, H论文数: 0 引用数: 0 h-index: 0机构: Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USA Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USAYe, ZH论文数: 0 引用数: 0 h-index: 0机构: Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USA Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USAZhan, XC论文数: 0 引用数: 0 h-index: 0机构: Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USA Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USADravid, G论文数: 0 引用数: 0 h-index: 0机构: Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USA Johns Hopkins Univ, Sch Med, Baltimore, MD 21231 USA
- [8] LIF/STAT3 signaling fails to maintain self-renewal of human embryonic stem cells[J]. STEM CELLS, 2004, 22 (05) : 770 - 778Dahéron, L论文数: 0 引用数: 0 h-index: 0机构: Harvard Univ, Sch Med, Div Pediat Hematol Oncol, Dept Biol Chem & Mol Pharmacol,Childrens Hosp, Boston, MA 02115 USAOpitz, SL论文数: 0 引用数: 0 h-index: 0机构: Harvard Univ, Sch Med, Div Pediat Hematol Oncol, Dept Biol Chem & Mol Pharmacol,Childrens Hosp, Boston, MA 02115 USAZaehres, H论文数: 0 引用数: 0 h-index: 0机构: Harvard Univ, Sch Med, Div Pediat Hematol Oncol, Dept Biol Chem & Mol Pharmacol,Childrens Hosp, Boston, MA 02115 USALensch, WM论文数: 0 引用数: 0 h-index: 0机构: Harvard Univ, Sch Med, Div Pediat Hematol Oncol, Dept Biol Chem & Mol Pharmacol,Childrens Hosp, Boston, MA 02115 USAAndrews, PW论文数: 0 引用数: 0 h-index: 0机构: Harvard Univ, Sch Med, Div Pediat Hematol Oncol, Dept Biol Chem & Mol Pharmacol,Childrens Hosp, Boston, MA 02115 USAItskovitz-Eldor, J论文数: 0 引用数: 0 h-index: 0机构: Harvard Univ, Sch Med, Div Pediat Hematol Oncol, Dept Biol Chem & Mol Pharmacol,Childrens Hosp, Boston, MA 02115 USADaley, GQ论文数: 0 引用数: 0 h-index: 0机构: Harvard Univ, Sch Med, Div Pediat Hematol Oncol, Dept Biol Chem & Mol Pharmacol,Childrens Hosp, Boston, MA 02115 USA
- [9] Culture and characterization of human embryonic stem cells[J]. STEM CELLS AND DEVELOPMENT, 2004, 13 (04) : 325 - 336Draper, JS论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandMoore, HD论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandRuban, LN论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandGokhale, PJ论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandAndrews, PW论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, England
- [10] Recurrent gain of chromosomes 17q and 12 in cultured human embryonic stem cells[J]. NATURE BIOTECHNOLOGY, 2004, 22 (01) : 53 - 54Draper, JS论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandSmith, K论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, England论文数: 引用数: h-index:机构:Moore, HD论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandMaltby, E论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, England论文数: 引用数: h-index:机构:Meisner, L论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandZwaka, TP论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandThomson, JA论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, EnglandAndrews, PW论文数: 0 引用数: 0 h-index: 0机构: Univ Sheffield, Dept Biomed Sci, Sheffield S10 2TN, S Yorkshire, England