Adult thymus contains dendritic epidermal T cell precursors that home to the epidermis in irradiated, but not in normal, mice

被引:5
作者
Hayakawa, J [1 ]
Moriya, N [1 ]
Shiohara, T [1 ]
机构
[1] KYORIN UNIV,SCH MED,DEPT DERMATOL,MITAKA,TOKYO 181,JAPAN
关键词
D O I
10.1006/cimm.1996.0309
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
It was generally assumed that V gamma 5(+) fetal thymocytes are the only cells capable of homing to the epidermis. However, we have recently demonstrated that reconstitution of thymectomized irradiated mice with T-cell-depleted bone marrow (BM) cells results in the appearance of donor-type CD8(+), TCR-alpha beta(+) dendritic epidermal T cells (DETC) phenotypically distinct from their normal gamma delta(+) counterpants, In the present study, we found that the epidermis of lethally irradiated, BM-reconstituted (XBM) mice was also repopulated by DETC of host origin as well as donor BM-derived DETC and that the host-derived DETC were rarely detected in the epidermis of athymic BM chimeras (ATXBM). This result indicates that the host-derived DETC are originated from intrathymic radioresistant cells that may share migratory capacities with the fetal thymocytes. We demonstrated that transfer of radioresistant intrathymic precursor cells into ATXBM mice resulted in the appearance of donor-type CD8(+), TCR-alpha beta(+) DETC, Contrary to our initial expectation, however, transferred normal adult thymocytes also gave rise to donor-type DETC with frequency similar to radioresistant thymocyte-derived DETC. These thymocyte-derived DETC were phenotypically similar to those derived hom BM cells but differed in some respects, Tissue tropism by the CD8(+) subset was observed only for the epidermis, but not for other lymphoid organs, such as lymph nodes. The epidermis of normal mice, unlike that of irradiated mice, was refractory to the appearance of the thymus-derived DETC. These results suggest that prior irradiation of the host may confer a susceptibility of the epidermis to repopulation of DETC precursors, which are otherwise incapable of migrating into the epidermis. This provides an explanation for why CD8(+), TCR-alpha beta(+) DETC of adult thymus or BM origin are rarely detected in the normal epidermis, but abundantly observed in the irradiated mice. (C) 1996 Academic Press, Inc.
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页码:190 / 198
页数:9
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