MicroRNAs (miRNAs) are ubiquitous regulators of eukaryotic gene expression. In addition to repressing translation, miRNAs can down-regulate the concentration of mRNAs that contain elements to which they are imperfectly complementary. Using miR-125b and let-7 as representative rill we show that in mammalian cells this reduction in message abundance is a consequence of accelerated deadenylation, which leads to rapid mRNA decay. The ability of miRNAs to expedite poly(A) removal does not result from decreased translation; nor does translational repression by miRNAs require a poly(A) tail, a 3' histone stem-loop being an effective substitute. These findings suggest that miRNAs use two distinct posttranscriptional mechanisms to down-regulate gene expression.
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Mourelatos, Z
Dostie, J
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Dostie, J
Paushkin, S
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Paushkin, S
Sharma, A
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Sharma, A
Charroux, B
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Charroux, B
Abel, L
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Abel, L
Rappsilber, J
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Rappsilber, J
Mann, M
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Mann, M
Dreyfuss, G
论文数: 0引用数: 0
h-index: 0
机构:
Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USAUniv Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Mourelatos, Z
Dostie, J
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Dostie, J
Paushkin, S
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Paushkin, S
Sharma, A
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Sharma, A
Charroux, B
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Charroux, B
Abel, L
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Abel, L
Rappsilber, J
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Rappsilber, J
Mann, M
论文数: 0引用数: 0
h-index: 0
机构:Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA
Mann, M
Dreyfuss, G
论文数: 0引用数: 0
h-index: 0
机构:
Univ Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USAUniv Penn, Sch Med, Howard Hughes Med Inst, Dept Biochem & Biophys, Philadelphia, PA 19104 USA