Flow-injection determination of L-histidine with an immobilized histidine oxidase from Brevibacillus borstelensis KAIT-B-022 and chemiluminescence detection

被引：9

作者：

Kiba, N

Koga, A

Tachibana, M

Tani, K

Koizumi, H

Koyama, T

Yamamura, A

Matsumoto, K ^{[1
]}

Okuda, T

Yokotsuka, K

机构：

[1] Kanagawa Inst Technol, Fac Engn, Dept Appl Chem, Atsugi, Kanagawa 2430292, Japan

[2] Univ Yamanashi, Fac Engn, Dept Appl Chem, Kofu, Yamanashi 4008511, Japan

[3] Univ Yamanashi, Lab Biofunct Sci, Inst Enol & Viticulture, Kofu, Yamanashi 4000005, Japan

来源：

ANALYTICAL SCIENCES | 2006年 / 22卷 / 01期

关键词：

D O I：

10.2116/analsci.22.95

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A chemiluminometric flow injection analytical system for the quantitation Of L-histidine is described. Histidine oxidase (EC 1.4.3.-) from Brevibacillus borstelensis KAIT-B-022 was immobilized on tresylated poly(vinyl alcohol) beads and packed into a stainless-steel column. The hydrogen peroxide produced was detected chemiluminometrically by a flow-through sensor containing immobilized peroxidase (EC 1.11.1.7). The maximum sample throughput was 10 h(-1). The calibration graph was linear from 0.05 to 5 mM; the detection limit (signal to noise ratio = 3) was 0.01 mM. The activity of immobilized histidine oxidase reduced to 65% of the initial value after 350 injections. The system was applied to the determination of L-histidine in fish meat, such as salmon, tunny, bonito, and mackerel.

引用

页码：95 / 98

页数：4

共 11 条

[1] HPLC method for analysis of free amino acids in fish using o-phthaldialdehyde precolumn derivatization [J].