Surface modification of poly(dimethylsiloxane) microfluidic devices and its application in simultaneous analysis of uric acid and ascorbic acid in human urine

被引：27

作者：

Liang, Ru-Ping ^{[1
,2
]}

Gan, Gui-Hua ^{[1
,2
]}

Qiu, Jian-Ding ^{[1
,2
,3
]}

机构：

[1] Nanchang Univ, Dept Chem, Nanchang 330031, Peoples R China

[2] Nanchang Univ, Inst Adv Study, Nanchang 330031, Peoples R China

[3] Pingxiang Coll, Dept Chem Engn, Pingxiang, Peoples R China

来源：

JOURNAL OF SEPARATION SCIENCE | 2008年 / 31卷 / 15期

关键词：

chitosan; deoxyribonucleic acid; layer-by-layer; modification; poly(dimethylsiloxane);

D O I：

10.1002/jssc.200800149

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel and simple method based on layer-by-layer (LBL) technique has been developed for the modification of the channel in PDMS electrophoresis microchip to create a hydrophilic Surface with a stable EOF. The functional surface was obtained by sequentially immobilizing chitosan and deoxyribonucleic acid (DNA) onto the microfluidic channel Surface using the LBL assembly technique. Compared to the native PDMS microchips, the contact angle of the chitosan-DNA modified PDMS microchips decreased and the EOF increased. Experimental conditions were optimized in detail. The chitosan-DNA modified PDMS microchips exhibited good reproducibility and long-term stability. Separation Of uric acid (UA) and ascorbic acid (AA) performed on the modified PDMS microchip generated 43450 and 46790 N/m theoretical plates compared with 4048 and 19 847 N/m with the native PDMS microchip. In addition, this method has been successfully applied to real human urine samples, without SPE, with recoveries of 97-105% for UA and AA.

引用

页码：2860 / 2867

页数：8

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