Lack of p21waf1 and p27kip1 protein induction by interferon-α2a in human melanoma cell lines

被引：5

作者：

Bearzatto, A ^{[1
]}

Orlandi, L ^{[1
]}

De Marco, C ^{[1
]}

Daidone, MG ^{[1
]}

Zaffaroni, N ^{[1
]}

机构：

[1] Ist Nazl Studio & Cura Tumori, Div Oncol Sperimentale C, I-20133 Milan, Italy

来源：

MELANOMA RESEARCH | 1999年 / 9卷 / 05期

关键词：

cell growth; cyclin-dependent kinases; interferon-alpha; 2a; STAT proteins;

D O I：

10.1097/00008390-199910000-00005

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The ability of human recombinant interferon-alpha 2a (IFN alpha 2a) to induce the expression of cyclin-dependent kinase inhibitors p21(waf1) and p27(kip1) consequent to signal transducers and activators of transcription (STAT) protein activation was investigated in six human melanoma cell lines with different susceptibilities to the antiproliferative effect of the cytokine. All the cell lines expressed IFN alpha and IFN alpha/beta receptors. Exposure for 24 h to IFN alpha 2a markedly enhanced the nuclear expression of STAT1 and STAT2 proteins in all the cell lines. However, no induction of p21(waf1) Or p27(kip1) was consistently observed. Overall, results from the study suggest that the induction of such cyclin-dependent kinase inhibitors is not a major mechanism for the antiproliferative effect of IFN alpha 2a, at least in human melanoma cell lines, (C) 1999 Lippincott Williams & Wilkins.

引用

页码：457 / 463

页数：7

共 24 条

[1]

ABRAMOVICH C, 1994, CYTOKINE, V6, P412

[2]

ANICHINI A, 1989, J IMMUNOL, V142, P3692

[3] Differential myeloma cell responsiveness to interferon-α correlates with differential induction of p19INK4d and cyclin D2 expression [J].

Arora, T ;

Jelinek, DF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (19) :11799-11805

[4]

Bae I, 1996, CANCER RES, V56, P840

[5] Combined effects of interferon-α2a and 13-cis-retinoic acid on human melanoma cell growth and STAT protein expression [J].

Bearzatto, A ;

Orlandi, L ;

Silvestrini, R ;

Belli, F ;

Cascinelli, N ;

Zaffaroni, N .

MELANOMA RESEARCH, 1998, 8 (01) :31-38

[6] Cell growth arrest and induction of cyclin-dependent kinase inhibitor p21(WAF1/CIP1) mediated by STAT1 [J].

Chin, YE ;

Kitagawa, M ;

Su, WCS ;

You, ZH ;

Iwamoto, Y ;

Fu, XY .

SCIENCE, 1996, 272 (5262) :719-722

[7] JAK-STAT PATHWAYS AND TRANSCRIPTIONAL ACTIVATION IN RESPONSE TO IFNS AND OTHER EXTRACELLULAR SIGNALING PROTEINS [J].

DARNELL, JE ;

KERR, IM ;

STARK, GR .

SCIENCE, 1994, 264 (5164) :1415-1421

[8]

ELDEIRY WS, 1994, CANCER RES, V54, P1169

[9] WAF1, A POTENTIAL MEDIATOR OF P53 TUMOR SUPPRESSION [J].

ELDEIRY, WS ;

TOKINO, T ;

VELCULESCU, VE ;

LEVY, DB ;

PARSONS, R ;

TRENT, JM ;

LIN, D ;

MERCER, WE ;

KINZLER, KW ;

VOGELSTEIN, B .

CELL, 1993, 75 (04) :817-825

[10] p21(Waf1/Cip1) protects against p53-mediated apoptosis of human melanoma cells [J].

Gorospe, M ;

Cirielli, C ;

Wang, XT ;

Seth, P ;

Capogrossi, MC ;

Holbrook, NJ .

ONCOGENE, 1997, 14 (08) :929-935

← 1 2 3 →