Lack of p21waf1 and p27kip1 protein induction by interferon-α2a in human melanoma cell lines

被引：5

作者：

Bearzatto, A ^{[1
]}

Orlandi, L ^{[1
]}

De Marco, C ^{[1
]}

Daidone, MG ^{[1
]}

Zaffaroni, N ^{[1
]}

机构：

[1] Ist Nazl Studio & Cura Tumori, Div Oncol Sperimentale C, I-20133 Milan, Italy

来源：

MELANOMA RESEARCH | 1999年 / 9卷 / 05期

关键词：

cell growth; cyclin-dependent kinases; interferon-alpha; 2a; STAT proteins;

D O I：

10.1097/00008390-199910000-00005

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The ability of human recombinant interferon-alpha 2a (IFN alpha 2a) to induce the expression of cyclin-dependent kinase inhibitors p21(waf1) and p27(kip1) consequent to signal transducers and activators of transcription (STAT) protein activation was investigated in six human melanoma cell lines with different susceptibilities to the antiproliferative effect of the cytokine. All the cell lines expressed IFN alpha and IFN alpha/beta receptors. Exposure for 24 h to IFN alpha 2a markedly enhanced the nuclear expression of STAT1 and STAT2 proteins in all the cell lines. However, no induction of p21(waf1) Or p27(kip1) was consistently observed. Overall, results from the study suggest that the induction of such cyclin-dependent kinase inhibitors is not a major mechanism for the antiproliferative effect of IFN alpha 2a, at least in human melanoma cell lines, (C) 1999 Lippincott Williams & Wilkins.

引用

页码：457 / 463

页数：7

共 24 条

[11] Economic analysis of adjuvant interferon alfa-2b in high-risk melanoma based on projections from eastern cooperative oncology group 1684 [J].

Hillner, BE ;

Kirkwood, JM ;

Atkins, MB ;

Johnson, ER ;

Smith, TJ .

JOURNAL OF CLINICAL ONCOLOGY, 1997, 15 (06) :2351-2358

[12] CYCLINS AND CANCER .2. CYCLIN-D AND CDK INHIBITORS COME OF AGE [J].

HUNTER, T ;

PINES, J .

CELL, 1994, 79 (04) :573-582

[13] Differential human multiple myeloma cell line responsiveness to interferon-alpha - Analysis of transcription factor activation and interleukin 6 receptor expression [J].

Jelinek, DF ;

AagaardTillery, KM ;

Arendt, BK ;

Arora, T ;

Tschumper, RC ;

Westendorf, JJ .

JOURNAL OF CLINICAL INVESTIGATION, 1997, 99 (03) :447-456

[14]

Kuniyasu H, 1997, CELL GROWTH DIFFER, V8, P47

[15] Regulation of STAT-dependent pathways by growth factors and cytokines [J].

Leaman, DW ;

Leung, S ;

Li, XX ;

Stark, GR .

FASEB JOURNAL, 1996, 10 (14) :1578-1588

[16] Interferon-α-induced G1 phase arrest through up-regulated expression of CDK inhibitors, p19Ink4D and p21Cip1 in mouse macrophages [J].

Matsuoka, M ;

Tani, K ;

Asano, S .

ONCOGENE, 1998, 16 (16) :2075-2086

[17] THE HUMAN INTERFERON-ALPHA/BETA RECEPTOR - CHARACTERIZATION AND MOLECULAR-CLONING [J].

NOVICK, D ;

COHEN, B ;

RUBINSTEIN, M .

CELL, 1994, 77 (03) :391-400

[18]

Oya M, 1997, INT J ONCOL, V10, P835

[19]

Platanias Leonidas C., 1995, Current Opinion in Oncology, V7, P560, DOI 10.1097/00001622-199511000-00015

[20] Induction of Cip/Kip and Ink4 cyclin dependent kinase inhibitors by interferon-alpha in hematopoietic cell lines [J].

Sangfelt, O ;

Erickson, S ;

Einhorn, S ;

Grander, D .

ONCOGENE, 1997, 14 (04) :415-423

← 1 2 3 →