Polyphenol oxidase activity staining in polyacrylamide electrophoresis gels

被引：25

作者：

Rescigno, A

Sollai, F

Rinaldi, AC

Soddu, G

Sanjust, E

机构：

[1] Istituto di Chimica Biologica, Università di Cagliari, I-09125 Cagliari

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1997年 / 34卷 / 02期

关键词：

polyphenol oxidase assay; catecholase assay; PAGE; 4-tert-butyl catechol;

D O I：

10.1016/S0165-022X(96)01201-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An analytical method allowing the detection of polyphenol oxidase activity on polyacrylamide gel electrophoresis (PAGE) is described. The method is rapid, sensitive and specific and is based on a coupling reaction between 4-tert-butyl-o-benzoquinone and the aromatic amine, 4-amino-N,N-diethylaniline sulphate. Catecholase activity of polyphenol oxidase appears as blue stained bands on a colourless background. (C) 1997 Elsevier Science B.V.

引用

页码：155 / 159

页数：5

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