A micro-nib nanoelectro spray source for mass spectrometry

被引:27
作者
Arscott, S
Le Gac, S
Druon, C
Tabourier, P
Rolando, C
机构
[1] Univ Sci & Tech Lille Flandres Artois, UMR CNRS 8520, IEMN, F-59652 Villeneuve Dascq, France
[2] Univ Lille 1, UMR CNRS 8009, LCOM, F-59655 Villeneuve Dascq, France
关键词
nano-electrospray ionisation; micro-fabrication; micro-fluidic device; lab-on-chip; mu TAS; SU-8; mass spectrometry; proteomics;
D O I
10.1016/j.snb.2003.10.002
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The design, fabrication and testing of a novel interface resembling a micro-nib for nano-electrospray ionisation-mass spectrometry (ESI-MS) applications is reported in this paper. The micro-nibs are fabricated in a planar topology using the negative photoresist SU-8 deposited onto silicon substrates. In order to direct the liquids to the nib tip, capillary slots with dimensions of 8 mum x 35 mum and 16 mum x 35 mum are included in the micro-nib design. The micro-nibs are fabricated so that they overhang from the edge of a silicon substrate in order to provide a reliable free-standing planar interface from a micro-fluidic circuit to a mass spectrometer. The micro-nib sources are tested on an ion trap mass spectrometer using standard peptide samples at concentrations down to 1 muM and with a typical ionisation voltage of around 1 kV. In addition, the nibs function well when the voltage supply was decreased to a value of 0.8kV. The resulting mass spectra are seen to have a good signal-to-noise ratio (S/N) comparable to those observed using classical emitter tips. The micro-nibs demonstrate state-of-the-art performances with respect to current micro-machined electrospray interfaces and are comparable to those obtained for stand-alone fused-silica-based emitter tips. (C) 2003 Elsevier B.V. All rights reserved.
引用
收藏
页码:140 / 147
页数:8
相关论文
共 24 条
[1]   Microfluidic devises connected to fused-silica capillaries with minimal dead volume [J].
Bings, NH ;
Wang, C ;
Skinner, CD ;
Colyer, CL ;
Thibault, P ;
Harrison, DJ .
ANALYTICAL CHEMISTRY, 1999, 71 (15) :3292-3296
[2]  
DESAI A, 1997, INT C SOL STAT SENS
[3]   A microfabricated device for rapid protein identification by microelectrospray ion trap mass spectrometry [J].
Figeys, D ;
Ning, YB ;
Aebersold, R .
ANALYTICAL CHEMISTRY, 1997, 69 (16) :3153-3160
[4]  
Gobry V, 2002, PROTEOMICS, V2, P405, DOI 10.1002/1615-9861(200204)2:4<405::AID-PROT405>3.0.CO
[5]  
2-Y
[6]   Development of micromachined hollow tips for protein analysis based on nanoelectrospray ionization mass spectrometry [J].
Griss, P ;
Melin, J ;
Sjödahl, J ;
Roeraade, J ;
Stemme, G .
JOURNAL OF MICROMECHANICS AND MICROENGINEERING, 2002, 12 (05) :682-687
[7]   An electrospray ionization source for integration with microfluidics [J].
Kameoka, J ;
Orth, R ;
Ilic, B ;
Czaplewski, D ;
Wachs, T ;
Craighead, HG .
ANALYTICAL CHEMISTRY, 2002, 74 (22) :5897-5901
[8]   Microfabricated PDMS multichannel emitter for electrospray ionization mass spectrometry [J].
Kim, JS ;
Knapp, DR .
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 2001, 12 (04) :463-469
[9]  
KOCH M, 2000, MICROFLUIDIC TECHNOL, P151
[10]  
LEGAC S, IN PRESS J AM SOC MA