Microfabricated PDMS multichannel emitter for electrospray ionization mass spectrometry

被引：92

作者：

Kim, JS ^{[1
]}

Knapp, DR ^{[1
]}

机构：

[1] Med Univ S Carolina, Dept Pharmacol, Charleston, SC 29425 USA

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 2001年 / 12卷 / 04期

基金：

美国国家科学基金会;

关键词：

D O I：

10.1016/S1044-0305(01)00219-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A novel microfabricated multichannel emitter for electrospray ionization mass spectrometry (ESI-MS) was implemented with polydimethylsiloxane (PDMS) using a soft lithography technique. The emitters are formed as electrospray tips along a thin membrane on the edge of the device with channels of 100 mum X 30 mum dimensions. The electrospray performance of the PDMS emitters for a single channel device and a four channel device interfaced with a time-of-flight mass spectrometer was evaluated for detecting the molecular weight of reference peptides (angiotensin I and bradykinin). The emitters were durable at the flow rate of 1-20 muL min-l for more than 30 h of continuous electrospray with limit of detection of 1 muM (S/N 18). This microfabrication method for a PDMS multichannel emitter as an integral part of a microfluidic device will facilitate development of more complex microfluidic analysis systems using ESI-MS. (C) 2001 American Society for Mass Spectrometry.

引用

页码：463 / 469

页数：7

共 28 条

[1] Fabrication of topologically complex three-dimensional microfluidic systems in PDMS by rapid prototyping [J].

Anderson, JR ;

Chiu, DT ;

Jackman, RJ ;

Cherniavskaya, O ;

McDonald, JC ;

Wu, HK ;

Whitesides, SH ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 2000, 72 (14) :3158-3164

[2] Microfabricated polymer devices for automated sample delivery of peptides for analysis by electrospray ionization tandem mass spectrometry [J].

Chan, JH ;

Timperman, AT ;

Qin, D ;

Aebersold, R .

ANALYTICAL CHEMISTRY, 1999, 71 (20) :4437-4444

[3]

DESAI A, 1997, 1997 INT C SOL STAT, P927

[4] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane) [J].

Duffy, DC ;

McDonald, JC ;

Schueller, OJA ;

Whitesides, GM .

ANALYTICAL CHEMISTRY, 1998, 70 (23) :4974-4984

[5] A microfabricated device for rapid protein identification by microelectrospray ion trap mass spectrometry [J].

Figeys, D ;

Ning, YB ;

Aebersold, R .

ANALYTICAL CHEMISTRY, 1997, 69 (16) :3153-3160

[6] Nanoflow solvent gradient delivery from a microfabricated device for protein identifications by electrospray ionization mass spectrometry [J].

Figeys, D ;

Aebersold, R .

ANALYTICAL CHEMISTRY, 1998, 70 (18) :3721-3727

[7] An integrated microfluidics tandem mass spectrometry system for automated protein analysis [J].

Figeys, D ;

Gygi, SP ;

McKinnon, G ;

Aebersold, R .

ANALYTICAL CHEMISTRY, 1998, 70 (18) :3728-3734

[8] Miniaturized total analysis systems for biological analysis [J].

Jakeway, SC ;

de Mello, AJ ;

Russell, EL .

FRESENIUS JOURNAL OF ANALYTICAL CHEMISTRY, 2000, 366 (6-7) :525-539

[9] Subattomole-sensitivity microchip nanoelectrospray source with time-of-flight mass spectrometry detection [J].

Lazar, IM ;

Ramsey, RS ;

Sundberg, S ;

Ramsey, JM .

ANALYTICAL CHEMISTRY, 1999, 71 (17) :3627-3631

[10] Integration of microfabricated devices to capillary electrophoresis-electrospray mass spectrometry using a low dead volume connection: Application to rapid analyses of proteolytic digests [J].

Li, JJ ;

Thibault, P ;

Bings, NH ;

Skinner, CD ;

Wang, C ;

Colyer, C ;

Harrison, J .

ANALYTICAL CHEMISTRY, 1999, 71 (15) :3036-3045

← 1 2 3 →