Multiplex PCR assay for detection of bacterial pathogens associated with warm-water streptococcosis in fish

被引：115

作者：

Mata, AI ^{[1
]}

Gibello, A ^{[1
]}

Casamayor, A ^{[1
]}

Blanco, MM ^{[1
]}

Domínguez, L ^{[1
]}

Fernández-Garayzábal, JF ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dpto Sanidad Anim, E-28040 Madrid, Spain

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2004年 / 70卷 / 05期

关键词：

D O I：

10.1128/AEM.70.5.3183-3187.2004

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A multiplex PCR-based method was designed for the simultaneous detection of the main pathogens involved in Warm-water streptococcosis in fish (Streptococcus iniae, Streptococcus difficilis, Sireptococcus paratiberis, and Lactococcus garvieae). Each of the four pairs of oligonucleotide primers exclusively amplified the targeted gene of the specific microorganism. The sensitivity of the multiplex PCR using purified DNA was 25 pg for S. iniae, 12.5 pg for S. difficilis, 50 pg for S. parauberis, and 30 pg for L. garvieae. The multiplex PCR assay was useful for the specific detection of the four species of bacteria not only in pure culture but also in inoculated fish tissue homogenates and naturally infected fish. Therefore, this method could be a useful alternative to the culture-based method for the routine diagnosis of warm-water streptococcal infections in fish.

引用

页码：3183 / 3187

页数：5

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