An electrochemical impedance immunoanalytical method for detecting immunological interaction of human mammary tumor associated glycoprotein and its monoclonal antibody

被引：42

作者：

Jie, M ^{[1
]}

Ming, CY

Jing, D

Cheng, LS

Na, LH

Jun, F

Xiang, CY

机构：

[1] Capital Normal Univ, Dept Chem, Beijing 100037, Peoples R China

[2] Peking Univ, Dept Chem, Beijing 100871, Peoples R China

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 1999年 / 1卷 / 09期

关键词：

electrochemical impedance spectroscopy; immunological reactions; immunosensors; antibody immobilization;

D O I：

10.1016/S1388-2481(99)00071-5

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

An electrochemical impedance immunosensor has been developed for the specific detection of immunological interaction between human mammary tumor associated glycoprotein and its monoclonal antibody (GP1D8). Antibody proteins were immobilized by spontaneous adsorption of antibody on gold. Consequently, electrochemical impedance spectroscopy (EIS) measurements of a gold electrode coated with the antibody showed changes in a.c. current response after the addition of the specific antigen. The successful immunological reaction between the immobilized antibody-antigen at the electrode surface could be monitored. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：425 / 428

页数：4

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