Full-Genome Deep Sequencing and Phylogenetic Analysis of Novel Human Betacoronavirus

被引：108

作者：

Cotten, Matthew ^{[1
]}

Lam, Tommy T. ^{[2
]}

Watson, Simon J. ^{[1
]}

Palser, Anne L. ^{[1
]}

Petrova, Velislava ^{[1
]}

Grant, Paul ^{[4
]}

Pybus, Oliver G. ^{[2
]}

Rambaut, Andrew ^{[5
,6
]}

Guan, Yi ^{[7
]}

Pillay, Deenan ^{[3
]}

Kellam, Paul ^{[1
,3
]}

Nastouli, Eleni ^{[4
]}

机构：

[1] Wellcome Trust Sanger Inst, Hinxton, England

[2] Univ Oxford, Oxford, England

[3] UCL, London, England

[4] Univ Coll London Hosp, London, England

[5] Univ Edinburgh, Edinburgh, Midlothian, Scotland

[6] NIH, Fogarty Int Ctr, Bethesda, MD 20892 USA

[7] Univ Hong Kong, Hong Kong, Hong Kong, Peoples R China

来源：

EMERGING INFECTIOUS DISEASES | 2013年 / 19卷 / 05期

基金：

英国惠康基金;

关键词：

HUMAN CORONAVIRUS OC43; SARS CORONAVIRUS; ALGORITHMS; DIVERSITY; ALIGNMENT; TIME; BATS;

D O I：

10.3201/eid1905.130057

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A novel betacoronavirus associated with lethal respiratory and renal complications was recently identified in patients from several countries in the Middle East. We report the deep genome sequencing of the virus directly from a patient's sputum sample. Our high-throughput sequencing yielded a substantial depth of genome sequence assembly and showed the minority viral variants in the specimen. Detailed phylogenetic analysis of the virus genome (England/Qatar/2012) revealed its close relationship to European bat coronaviruses circulating among the bat species of the Vespertilionidae family. Molecular clock analysis showed that the 2 human infections of this betacoronavirus in June 2012 (EMC/2012) and September 2012 (England/Qatar/2012) share a common virus ancestor most likely considerably before early 2012, suggesting the human diversity is the result of multiple zoonotic events.

引用

页码：736 / 742

页数：7

共 31 条

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