Antioxidant activity of sugarcane molasses against 2,2′-azobis(2-amidinopropane) dihydrochloride-induced peroxyl radicals

被引：50

作者：

Asikin, Yonathan ^{[1
]}

Takahashi, Makoto ^{[2
]}

Mishima, Takashi ^{[3
]}

Mizu, Masami ^{[4
]}

Takara, Kensaku ^{[1
,2
]}

Wada, Koji ^{[1
,2
]}

机构：

[1] Kagoshima Univ, United Grad Sch Agr Sci, Kagoshima 8900065, Japan

[2] Univ Ryukyus, Fac Agr, Dept Biosci & Biotechnol, Nishihara, Okinawa 9030213, Japan

[3] Japan Food Res Labs, Div Res & Dev, Osaka 5670085, Japan

[4] Mitsui Sugar Co Ltd, Prod Dev Div, Chuo Ku, Tokyo 1038423, Japan

来源：

FOOD CHEMISTRY | 2013年 / 141卷 / 01期

基金：

日本学术振兴会;

关键词：

Sugarcane molasses; Peroxyl radicals; Chemical and cellular antioxidants; DNA damage protection; Phenolic constituents; CANE SUGAR; PHENOLIC COMPOSITION; SCAVENGING ACTIVITY; OFFICINARUM L; DNA; CONSTITUENTS; PRODUCTS; DAMAGE; PEROXIDATION; GLYCOSIDES;

D O I：

10.1016/j.foodchem.2013.03.045

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Sugarcane molasses is a rich source of antioxidant materials with peroxyl radical scavenging effects. To explore the potent antioxidant activity of sugarcane molasses against 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH)-induced peroxyl radicals, 7 methanolic fractions of sugarcane molasses (F1-F7) were separated via bioassay-guided fractionation and evaluated by oxygen radical absorbance capacity (ORAC), cellular antioxidant activity (CAA), and oxidative DNA damage protective activity assays. The ORAC values of sugarcane molasses fractions ranged from 4399 to 6266 mu mol TE/g, whilst the EC50 values for CAA ranged from 3.7 to 5.9 mu g/ml. Moreover, it was found that sugarcane molasses fractions, particularly F6 and F7, could protect against oxidative DNA damage caused by peroxyl radicals at an effective concentration of 100 mu g/ml. Ten phenolic constituents were identified in the fractions, including known antioxidative compounds, viz., schaftoside, isoschaftoside, ferulic acid, p-coumaric acid, and p-hydroxybenzaldehyde. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：466 / 472

页数：7

共 30 条

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p-Coumaric Acid Not Only Inhibits Human Tyrosinase Activity In Vitro but Also Melanogenesis in Cells Exposed to UVB [J].