Electrochemical biosensor based on hairpin DNA probe using 2-nitroacridone as electrochemical indicator for detection of DNA species related to Chronic Myelogenous Leukemia

被引：25

作者：

Chen, Jinghua ^{[1
,2
,3
]}

Zhang, Jing ^{[1
,2
,4
]}

Wang, Kun ^{[3
]}

Huang, Liying ^{[1
,2
,3
]}

Lin, Xinhua ^{[3
]}

Chen, Guonan ^{[1
,2
]}

机构：

[1] Fuzhou Univ, Minist Educ, Key Lab Anal & Detect Food Safety, Fuzhou 350002, Fujian, Peoples R China

[2] Fuzhou Univ, Dept Chem, Fuzhou 350002, Fujian, Peoples R China

[3] Fujian Med Univ, Fac Pharm, Dept Pharmaceut Anal, Fuzhou 350004, Peoples R China

[4] Fujian Coll Med Occupat & Technol, Dept Pharmaceut, Fuzhou 350101, Peoples R China

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 2008年 / 10卷 / 10期

基金：

中国国家自然科学基金;

关键词：

Electrochemical biosensor; Hairpin DNA probe; 2-Nitroacridone; BCR/ABL fusion gene; Chronic Myelogenous Leukemia;

D O I：

10.1016/j.elecom.2008.07.035

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

In this article, a new kind of hairpin DNA Electrochemical biosensor using nitroacridone as electrochemical indicator was first designed, and used to detect BCR/ABL fusion gene in Chronic Myelogenous Leukemia (CML). The results indicated that in pH 7.0 Tris-HCl buffer solution, the oxidation peak current was linear with the concentration of complementary strand in the range of 6.2 x 10(-8)-3.1 x 10(-7) mol/l with a detection limit of 5.3 x 10(-9) mol/l. This new hairpin DNA electrochemical biosensor demonstrates its excellent specificity for single-base mismatch and complementary (dsDNA) after hybridization, and this probe has been used for assay of PCR product of a real sample with satisfactory result. (c) 2008 Elsevier B.V. All rights reserved.

引用

页码：1448 / 1451

页数：4

共 17 条

[1] Fluorescence in situ hybridization: a highly efficient technique of molecular diagnosis and predication for disease course in patients with myeloid leukemias [J].

Amare, PS ;

Baisane, C ;

Saikia, T ;

Nair, R ;

Gawade, H ;

Advani, S .

CANCER GENETICS AND CYTOGENETICS, 2001, 131 (02) :125-134

[2] BCR/ABL and leukemia [J].

Butturini, A ;

Arlinghaus, RB ;

Gale, RP .

LEUKEMIA RESEARCH, 1996, 20 (06) :523-529

[3]

CHAMPLIN RE, 1985, BLOOD, V65, P1039

[4]

Chen JH, 2005, CHINESE J ANAL CHEM, V33, P1607

[5] Chronic myelogenous leukemia: A review [J].

Cortes, JE ;

Talpaz, M ;

Kantarjian, H .

AMERICAN JOURNAL OF MEDICINE, 1996, 100 (05) :555-570

[6] The amount of BCR-ABL fusion transcripts detected by the real-time quantitative polymerase chain reaction method in patients with Philadelphia chromosome positive chronic myeloid leukemia correlates with the disease stage [J].

Elmaagacli, AH ;

Beelen, DW ;

Opalka, B ;

Seeber, S ;

Schafer, UW .

ANNALS OF HEMATOLOGY, 2000, 79 (08) :424-431

[7] Novel hybridization indicator methylene blue for the electrochemical detection of short DNA sequences related to the hepatitis B virus [J].

Erdem, A ;

Kerman, K ;

Meric, B ;

Akarca, US ;

Ozsoz, M .

ANALYTICA CHIMICA ACTA, 2000, 422 (02) :139-149

[8] CLONAL ORIGIN OF CHRONIC MYELOCYTIC LEUKEMIA IN MAN [J].

FIALKOW, PJ ;

GARTLER, SM ;

YOSHIDA, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1967, 58 (04) :1468-&

[9] Identification of PML-RARA rearrangement by RT-PCR and sequencing in an acute promyelocytic leukemia without t(15;17) on G-banding and FISH [J].

Han, Jin-Yeong ;

Kim, Kyong-Eun ;

Kim, Kyeong-Hee ;

Park, Joo-In ;

Kim, Jae-Seok .

LEUKEMIA RESEARCH, 2007, 31 (02) :239-243

[10] Inhibition of K562 leukemia angiogenesis and growth by expression of antisense vascular endothelial growth factor (VEGF) sequence [J].

He, R ;

Liu, B ;

Yang, C ;

Yang, RC ;

Tobelem, G ;

Han, ZC .

CANCER GENE THERAPY, 2003, 10 (12) :879-886

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