The effect of hair color on the incorporation of methadone into hair in the rat

Six groups of six male, hooded, Lister rats were administered methadone in their drinking water over the concentration range 0-0.25 mg/mL for 6 weeks. Black pigmented and white nonpigmented hair and trunk blood samples were collected. Plasma and alkali digests of hair were analyzed for methadone by radioimmunoassay. Hair melanin content was estimated in digests by turbidimetry. Oral methadone intake rose in a linear fashion over the six dose groups, and plasma methadone concentration followed a similar trend. The methadone content of both white and black hair increased over the six dose groups, but concentrations were significantly higher in black compared with white hair. The mean ratio of methadone concentration between black and white hair was 21.3:1, and the mean ratio in hair melanin content was 3.5:1. The results demonstrate that pigmented hair incorporates larger quantities of methadone than nonpigmented hair and that methadone binds with higher affinity to the pigmented components as compared with other components of hair.