Promoter DNA Hypermethylation and Gene Repression in Undifferentiated Arabidopsis Cells

被引：95

作者：

Berdasco, Maria ^{[1
,2
,3
]}

Alcazar, Ruben ^{[4
]}

Victoria Garcia-Ortiz, Maria ^{[5
]}

Ballestar, Esteban ^{[1
]}

Fernandez, Agustin F. ^{[1
]}

Roldan-Arjona, Teresa ^{[5
]}

Tiburcio, Antonio F. ^{[4
]}

Altabella, Teresa ^{[4
]}

Buisine, Nicolas ^{[6
]}

Quesneville, Hadi ^{[7
]}

Baudry, Antoine ^{[8
]}

Lepiniec, Loic ^{[8
]}

Alaminos, Miguel ^{[9
]}

Rodriguez, Roberto ^{[2
]}

Lloyd, Alan ^{[10
]}

Colot, Vincent ^{[6
]}

Bender, Judith ^{[11
]}

Jesus Canal, Maria ^{[2
]}

Esteller, Manel ^{[1
,3
]}

Fraga, Mario F. ^{[1
,12
]}

机构：

[1] Spanish Natl Canc Ctr CNIO, Mol Pathol Programme, Canc Epigenet Lab, Madrid, Spain

[2] Univ Oviedo, Plant Epigenet Lab, Oviedo, Spain

[3] Catalan Inst Oncol ICO, Canc Epigenet & Biol Program PEBC, Barcelona, Spain

[4] Univ Barcelona, Fac Farm, Unitat Fisiol Vegetal, E-08007 Barcelona, Spain

[5] Univ Cordoba, Dept Genet, Cordoba, Spain

[6] Univ Evry Val Essonne, INRA, UMR1165, CNRS, Unite Rech Genom Vegetale, Evry, France

[7] INRA, Unite Rech Genom Info, Evry, France

[8] Inst Natl Rech Agronom 204, Unite Mixte Rech, Lab Biol Semences, Versailles, France

[9] Univ Granada, Dept Histol, E-18071 Granada, Spain

[10] Univ Texas, Inst Cellular & Mol Biol, Austin, TX USA

[11] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Biochem & Mol Biol, Baltimore, MD 21218 USA

[12] Ctr Nacl Biotecnol, CSIC, Dept Immunol & Oncol, Madrid, Spain

来源：

PLOS ONE | 2008年 / 3卷 / 10期

关键词：

D O I：

10.1371/journal.pone.0003306

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Maintaining and acquiring the pluripotent cell state in plants is critical to tissue regeneration and vegetative multiplication. Histone-based epigenetic mechanisms are important for regulating this undifferentiated state. Here we report the use of genetic and pharmacological experimental approaches to show that Arabidopsis cell suspensions and calluses specifically repress some genes as a result of promoter DNA hypermethylation. We found that promoters of the MAPK12, GSTU10 and BXL1 genes become hypermethylated in callus cells and that hypermethylation also affects the TTG1, GSTF5, SUVH8, fimbrin and CCD7 genes in cell suspensions. Promoter hypermethylation in undifferentiated cells was associated with histone hypoacetylation and primarily occurred at CpG sites. Accordingly, we found that the process specifically depends on MET1 and DRM2 methyltransferases, as demonstrated with DNA methyltransferase mutants. Our results suggest that promoter DNA methylation may be another important epigenetic mechanism for the establishment and/or maintenance of the undifferentiated state in plant cells.

引用

页数：10

共 46 条

[1] Reorganization of specific chromosomal domains and activation of silent genes in plant cells acquiring pluripotentiality [J].