A double-strand break repair component is essential for S phase completion in fission yeast cell cycling

被引:39
作者
Suto, K [1 ]
Nagata, A [1 ]
Murakami, H [1 ]
Okayama, H [1 ]
机构
[1] Univ Tokyo, Grad Sch Med, Dept Biochem & Mol Biol, Tokyo 1130033, Japan
关键词
D O I
10.1091/mbc.10.10.3331
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fission yeast rad22(+), a homologue of budding yeast RAD52, encodes a double-strand break repair component, which is dispensable for proliferation. We, however, have recently obtained a cell division cycle mutant with a temperature-sensitive allele of rad22(+), designated rad22-H6, which resulted from a point mutation in the conserved coding sequence leading to one amino acid alteration. We have subsequently isolated rad22(+) and its novel homologue rti1(+) as multicopy suppressors of this mutant, rti1(+) suppresses all the defects of cells lacking rad22(+). Mating type switch-inactive heterothallic cells lacking either rad22(+) or rti1(+) are viable, but those lacking both genes are inviable and arrest proliferation with a cell division cycle phenotype. At the nonpermissive temperature, a synchronous culture of rad22-H6 cells performs DNA synthesis without delay and arrests with chromosomes seemingly intact and replication completed and with a high level of tyrosine-phosphorylated Cdc2. However, rad22-H6 cells show a typical S phase arrest phenotype if combined with the rad1-1 checkpoint mutation. rad22(+) genetically interacts with rad11(+), which encodes the large subunit of replication protein A. Deletion of rad22(+)/rti1(+) or the presence of rad22-H6 mutation decreases the restriction temperature of rad11-A1 cells by 4-6 degrees C and leads to cell cycle arrest with chromosomes incompletely replicated. Thus, in fission yeast a double-strand break repair component is required for a certain step of chromosome replication unlinked to repair, partly via interacting with replication protein A.
引用
收藏
页码:3331 / 3343
页数:13
相关论文
共 54 条
[21]   THE FISSION YEAST CDC18+ GENE-PRODUCT COUPLES S-PHASE TO START AND MITOSIS [J].
KELLY, TJ ;
MARTIN, GS ;
FORSBURG, SL ;
STEPHEN, RJ ;
RUSSO, A ;
NURSE, P .
CELL, 1993, 74 (02) :371-382
[22]   EFFECTS OF BLEOMYCIN ON GROWTH-KINETICS AND SURVIVAL OF SACCHAROMYCES-CEREVISIAE - A MODEL OF REPAIR PATHWAYS [J].
KESZENMAN, DJ ;
SALVO, VA ;
NUNES, E .
JOURNAL OF BACTERIOLOGY, 1992, 174 (10) :3125-3132
[23]  
Lim DS, 1996, MOL CELL BIOL, V16, P7133
[24]   GENETIC-ANALYSIS OF GAMMA-RAY MUTAGENESIS IN YEAST .3. DOUBLE-MUTANT STRAINS [J].
MCKEE, RH ;
LAWRENCE, CW .
MUTATION RESEARCH, 1980, 70 (01) :37-48
[25]  
MORENO S, 1991, METHOD ENZYMOL, V194, P795
[26]   DNA strand annealing is promoted by the yeast Rad52 protein [J].
Mortensen, UH ;
Bendixen, C ;
Sunjevaric, I ;
Rothstein, R .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (20) :10729-10734
[27]   Homologous recombination in the fission yeast Schizosaccharomyces pombe: Different requirements for the rhp51(+), rhp54(+) and rad22(+) genes [J].
Muris, DFR ;
Vreeken, K ;
Schmidt, H ;
Ostermann, K ;
Clever, B ;
Lohman, PHM ;
Pastink, A .
CURRENT GENETICS, 1997, 31 (03) :248-254
[28]   CLONING THE RAD51 HOMOLOG OF SCHIZOSACCHAROMYCES-POMBE [J].
MURIS, DFR ;
VREEKEN, K ;
CARR, AM ;
BROUGHTON, BC ;
LEHMANN, AR ;
LOHMAN, PHM ;
PASTINK, A .
NUCLEIC ACIDS RESEARCH, 1993, 21 (19) :4586-4591
[29]   CLONING OF HUMAN AND MOUSE GENES HOMOLOGOUS TO RAD52, A YEAST GENE INVOLVED IN DNA-REPAIR AND RECOMBINATION [J].
MURIS, DFR ;
BEZZUBOVA, O ;
BUERSTEDDE, JM ;
VREEKEN, K ;
BALAJEE, AS ;
OSGOOD, CJ ;
TROELSTRA, C ;
HOEIJMAKERS, JHJ ;
OSTERMANN, K ;
SCHMIDT, H ;
NATARAJAN, AT ;
EEKEN, JCJ ;
LOHMAN, PHM ;
PASTINK, A .
MUTATION RESEARCH-DNA REPAIR, 1994, 315 (03) :295-305
[30]   Characterization of strand exchange activity of yeast Rad51 protein [J].
Namsaraev, E ;
Berg, P .
MOLECULAR AND CELLULAR BIOLOGY, 1997, 17 (09) :5359-5368