Pollen tube development and competitive ability are impaired by disruption of a Shaker K+ channel in Arabidopsis

被引:172
作者
Mouline, K
Véry, AA
Gaymard, F
Boucherez, J
Pilot, G
Devic, M
Bouchez, D
Thibaud, JB
Sentenac, H [1 ]
机构
[1] INRA, CNRS, UM2 Agro M, UMR 5004, F-34060 Montpellier 1, France
[2] UMP, CNRS, UMR 5096, Lab Genome & Dev Plantes, F-66860 Perpignan, France
[3] INRA, Ctr Versailles, Stn Genet & Ameliorat Plantes, F-78026 Versailles, France
关键词
pollen tube development; Shaker K+ channel; Arabidopsis; T-DNA knockout mutant;
D O I
10.1101/gad.213902
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Sexual reproduction in plants requires elongation of the pollen tube through the transmitting tissues toward the ovary. Tube growth rate is a major determinant of pollen competitive ability. We report that a K+ channel of the Shaker family in Arabidopsis, SPIK, plays an important role in pollen tube development. SPIK was found to be specifically expressed in pollen. When SPIK was heterologously expressed in COS cells, its product formed hyperpolarization-activated K+ channels. Disruption (T-DNA insertion) of the SPIK coding sequence strongly affected inwardly rectifying K+-channel activity in the pollen-grain plasma membrane. Measurements of membrane potential in growing pollen tubes yielded data compatible with a contribution of SPIK to K+ influx. In vitro pollen germination assays were performed, revealing that the disruption results in impaired pollen tube growth. Analysis of the transmission rate of the disrupted allele in the progeny of heterozygous plants revealed a decrease in pollen competitive ability, the probability of fertilization by mutant pollen being similar to1.6 times lower than that by wild-type pollen. The whole set of data supports the hypothesis that functional expression of SPIK plays a role in K+ uptake in the growing pollen tube, and thereby in tube development and pollen competitive ability.
引用
收藏
页码:339 / 350
页数:12
相关论文
共 71 条
[1]   POPULATION GENETIC-STRUCTURE AND OUTCROSSING RATE OF ARABIDOPSIS-THALIANA (L) HEYNH [J].
ABBOTT, RJ ;
GOMES, MF .
HEREDITY, 1989, 62 :411-418
[2]   GORK, a delayed outward rectifier expressed in guard cells of Arabidopsis thaliana, is a K+-selective, K+-sensing ion channel [J].
Ache, P ;
Becker, D ;
Ivashikina, N ;
Dietrich, P ;
Roelfsema, MRG ;
Hedrich, R .
FEBS LETTERS, 2000, 486 (02) :93-98
[3]   THE GENE FAMILY ENCODING THE ARABIDOPSIS-THALIANA TRANSLATION ELONGATION-FACTOR EF-1-ALPHA - MOLECULAR-CLONING, CHARACTERIZATION AND EXPRESSION [J].
AXELOS, M ;
BARDET, C ;
LIBOZ, T ;
LEVANTHAI, A ;
CURIE, C ;
LESCURE, B .
MOLECULAR & GENERAL GENETICS, 1989, 219 (1-2) :106-112
[4]   CHANGES IN POTASSIUM-ION CONCENTRATIONS DURING POLLEN DEHYDRATION AND GERMINATION IN RELATION TO PROTEIN-SYNTHESIS [J].
BASHE, D ;
MASCARENHAS, JP .
PLANT SCIENCE LETTERS, 1984, 35 (01) :55-60
[5]  
BECHTOLD N, 1993, CR ACAD SCI III-VIE, V316, P1194
[6]  
BELLANDO M, 1989, PLANT MEMBRANE TRANS, P75
[7]   The turgor pressure of growing lily pollen tubes [J].
Benkert, R ;
Obermeyer, G ;
Bentrup, FW .
PROTOPLASMA, 1997, 198 (1-2) :1-8
[8]   Functional comparison of plant inward-rectifier channels expressed in yeast [J].
Bertl, A ;
Reid, JD ;
Sentenac, H ;
Slayman, CL .
JOURNAL OF EXPERIMENTAL BOTANY, 1997, 48 :405-413
[9]  
BOUCHEZ D, 1993, CR ACAD SCI III-VIE, V316, P1188
[10]   The S-locus receptor kinase is inhibited by thioredoxins and activated by pollen coat proteins [J].
Cabrillac, D ;
Cock, JM ;
Dumas, C ;
Gaude, T .
NATURE, 2001, 410 (6825) :220-223