Sargachromanol G inhibits osteoclastogenesis by suppressing the activation NF-κB and MAPKs in RANKL-induced RAW 264.7 cells

被引:38
作者
Yoon, Weon-Jong [1 ,4 ]
Kim, Kil-Nam [2 ]
Heo, Soo-Jin [3 ]
Han, Sang-Chul [1 ]
Kim, Jihyeon [4 ]
Ko, Yeong-Jong [4 ]
Kang, Hee-Kyoung [1 ]
Yoo, Eun-Sook [1 ]
机构
[1] Jeju Natl Univ, Sch Med, Cheju 690756, South Korea
[2] Korea Basic Sci Inst KBSI, Marine Bio Res Team, Cheju 690756, South Korea
[3] Korea Inst Ocean Sci & Technol, Global Bioresources Res Ctr, Ansan 426744, South Korea
[4] Jeju TECHNOPK JTP, JBRI, Cheju 699943, South Korea
关键词
RANKL; Osteoclastogenesis; Sagachromanol G (SG); Osteoclastogenic marker gene; MARK; NF-kappa B; RECEPTOR ACTIVATOR; C-JUN; DIFFERENTIATION; EXPRESSION; SARGASSUM; OSTEOPETROSIS; FAMILY; GENE; FOS; ERK;
D O I
10.1016/j.bbrc.2013.04.046
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
070307 [化学生物学]; 071010 [生物化学与分子生物学];
摘要
Inflammatory cytokines play a major role in osteoclastogenesis, leading to the bone resorption that is frequently associated with osteoporosis. Sargachromanol G (SG), isolated from the brown alga Sargassum siliquastrum, inhibits the production of inflammatory cytokines. In the present study, we determined the effect of SG on receptor activator of NF-kappa B ligand (RANKL)-induced osteoclast formation. SG inhibited RANKL-induced osteoclast differentiation from RAW264.7 cells without signs of cytotoxicity. Additionally, the expression of osteoclastic marker genes, such as tartrate-resistant acid phosphatase (TRAP), cathepsin K (CTSK), matrix metalloproteinase 9 (MMP9), and calcitonin receptor (CTR), was strongly inhibited. SG inhibited RANKL-induced activation of NF-kappa B by suppressing RANKL-mediated I kappa B-alpha, degradation. Furthermore, SG inhibited RANKL-induced phosphorylation of mitogen activated protein kinases (p38, JNK, and ERK). This study identified SG as an inhibitor for osteoclast formation and provided evidence that natural compounds, such as SG, are alternative medicines for preventing and treating osteolysis. (C) 2013 Elsevier Inc. All rights reserved.
引用
收藏
页码:892 / 897
页数:6
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