Molecular diversity of pMMO and sMMO in a TCE-contaminated aquifer during bioremediation

被引：16

作者：

Baker, PW ^{[1
]}

Futamata, H ^{[1
]}

Harayama, S ^{[1
]}

Watanabe, K ^{[1
]}

机构：

[1] Marine Biotechnol Inst, Kamaishi, Iwate 0260001, Japan

来源：

FEMS MICROBIOLOGY ECOLOGY | 2001年 / 38卷 / 2-3期

关键词：

methanotroph; methane monooxygenase; competitive polymerase chain reactions trichloroethylene; methane biostimulation;

D O I：

10.1111/j.1574-6941.2001.tb00894.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The particulate methane monooxygenase genes (pMMO.) were amplified from methane-biostimulated aquifer samples using two sets of primers, A 189 plus A682 and pmof2 plus pmor, and the products were cloned and sequenced, The analysis of the sequences revealed a high diversity of pMMO genes in the aquifers. Some of the pMMO gene sequences (LP20 and LP21) isolated in this study were unique and unrelated to methanotrophs previously isolated from this site. Throughout all the samples collected from the different methane biostimulation trials, competitive PCR amplification using specific pMMO primers revealed that type I methanotrophs predominated over type 11 methanotrophs. The partial soluble methane monooxygenase genes were also amplified from the aquifer samples using specially designed primers but sequencing revealed a lower diversity. (C) 2001 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：161 / 167

页数：7

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