Intracellular uptake and release of poly (ethyleneimine)-co-poly(methyl methacrylate) nanoparticle/pDNA complexes for gene delivery

被引：43

作者：

Feng, M ^{[1
]}

Lee, D ^{[1
]}

Li, P ^{[1
]}

机构：

[1] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Open Lab Chirotechnol, Inst Mol Technol Drug Discovery & Synth, Kowloon, Hong Kong, Peoples R China

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2006年 / 311卷 / 1-2期

关键词：

cationic PEI-PMMA nanoparticles; nanoparticle/pDNA complexes; intracellular behavior; confocal laser scanning microscopy;

D O I：

10.1016/j.ijpharm.2005.12.035

中图分类号：

R9 [药学];

学科分类号：

1007 [药学];

摘要：

Our previous studies demonstrated that cationic nanoparticles composed of well-defined poly(methyl methacrylate) (PMMA) cores surrounded by a hairly poly(ethyleneimine) (PEI) shells have comparative advantages over the PEI system for gene delivery. In this study, we focused on the intracellular uptake and release of PEI-PMMA natioparticle/pDNA complexes. The behavior of the nanoparticle/pDNA complexes in recipient cells was monitored by using confocal laser scanning microscopy. We found that the nanoparticle/pDNA complexes were internalized very effectively by endocytosis. In the recipient cells the nanoparticles were found localized in the cytoplasm. At the same time, the pDNA carried by the nanoparticles successfully detached from the nanoparticles and localized in the nucleus of the HeLa cells. (c) 2006 Elsevier B.V. All rights reserved.

引用

页码：209 / 214

页数：6

共 19 条

[1]

Regulation of in vitro gene expression using antisense oligonucleotides or antisense expression plasmids transfected using starburst PAMAM dendrimers [J].