IFN-γ and IP-10 in Tracheal Aspirates From Premature Infants: Relationship With Bronchopulmonary Dysplasia

Background: Interferon-gamma (IFN-gamma) and interferon-inducible protein of 10 kDa (IP-10) are potent inflammatory mediators and contribute to acute lung injury in adults. Recently, a potential role for IFN-gamma and IP-10 in the pathogenesis of bronchopulmonary dysplasia (BPD) has been reported in animal models. Objective: To study the association between IFN-gamma and IP-10 in tracheal aspirate (TA) and the development of BPD in premature infants. Design/Methods: TA samples collected within 48 hr after birth from 79 mechanically ventilated premature neonates [gestational age (GA) <30 weeks (w), birth weight (BW) <1,250 g (g)] were analyzed. IFN-gamma was measured in a subgroup of 38 infants by using a biochip multi-analyte immunoassay. The level of IP-10 was determined using a commercially available ELISA kit. Total protein in TA was measured by Bradford assay to correct for sampling related dilution. BPD was defined as the need of supplemental oxygen at 36 weeks postmenstrual age (PMA). Results: Twenty infants (GA 26.4 +/- 1.9w, BW 860 +/- 201 g) survived without BPD at 36 weeks PMA and 59 infants (GA 25.5 +/- 1.5w, BW 751 +/- 163 g) died before 36 weeks PMA or developed BPD. The mean IFN-gamma level was higher in infants who died or developed BPD (9.7 +/- 2.8 vs. 3.1 +/- 1.1 pg/ml, P = 0.03). Similarly, the mean IP-10 level was higher in infants who died or developed BPD (63.4 +/- 17.5 pg/ml) compared to those who survived without BPD (18.5 +/- 7.5 pg/ml, P = 0.02). Conclusions: Higher IFN-gamma and IP-10 levels in TA samples are associated with the development of BPD or death in premature infants. Pediatr Pulmonol. 2013; 48:8-13. (C) 2012 Wiley Periodicals, Inc.