A simple procedure for constructing 5'-amino-terminated oligodeoxynucleotides in aqueous solution

A rapid method for the synthesis of oligodeoxynucleotides (ODNs) terminated by 5'-amino-5'-deoxythymidine is described, A 9'-phosphorylated ODN (the donor) is incubated in aqueous solution with 5'-amino-5'-deoxythymidine in the presence of N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC), extending the donor by one residue via a phosphoramidate bond, Template- directed ligation of the extended donor and an acceptor ODN, followed by acid hydrolysis, yields the acceptor ODN extended by a single 5'-amino-5'-deoxythymidine residue at its 5'terminus.