PREPARATION OF OLIGONUCLEOTIDE PEPTIDE CONJUGATES

被引：51

作者：

TUNG, CH

RUDOLPH, MJ

STEIN, S

机构：

[1] RUTGERS STATE UNIV,CTR ADV BIOTECHNOL & MED,679 HOES LANE,NEW BRUNSWICK,NJ 08903

[2] RUTGERS STATE UNIV,DEPT CHEM,NEW BRUNSWICK,NJ 08903

来源：

BIOCONJUGATE CHEMISTRY | 1991年 / 2卷 / 06期

关键词：

D O I：

10.1021/bc00012a015

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for preparing oligonucleotide-peptide conjugates is presented. It is based on appending a maleimide group to the oligonucleotide for selective coupling to the thiol side chain of a cysteine residue in the peptide. A convenient chromatographic purification procedure, based on Fmoc-on/Fmoc-off, is described.

引用

收藏

页码：464 / 465

页数：2

相关论文

共 13 条

[1] SITE SPECIFIC FUNCTIONALIZATION OF OLIGONUCLEOTIDES FOR ATTACHING 2 DIFFERENT REPORTER GROUPS [J].

AGRAWAL, S ;

ZAMECNIK, PC .

NUCLEIC ACIDS RESEARCH, 1990, 18 (18) :5419-5423

[2] LIGATION OF OLIGONUCLEOTIDES TO NUCLEIC-ACIDS OR PROTEINS VIA DISULFIDE BONDS [J].

CHU, BCF ;

ORGEL, LE .

NUCLEIC ACIDS RESEARCH, 1988, 16 (09) :3671-3691

[3] SYNTHESIS OF DEFINED PEPTIDE-OLIGONUCLEOTIDE HYBRIDS CONTAINING A NUCLEAR TRANSPORT SIGNAL SEQUENCE [J].

ERITJA, R ;

PONS, A ;

ESCARCELLER, M ;

GIRALT, E ;

ALBERICIO, F .

TETRAHEDRON, 1991, 47 (24) :4113-4120

[4] A SIMPLE METHOD FOR THE INTRODUCTION OF THIOL-GROUP AT 5'-TERMINI OF OLIGODEOXYNUCLEOTIDES [J].

GAUR, RK ;

SHARMA, P ;

GUPTA, KC .

NUCLEIC ACIDS RESEARCH, 1989, 17 (11) :4404-4404

[5] Use of Maleimide-Thiol Coupling Chemistry for Efficient Syntheses of Oligonucleotide-Enzyme Conjugate Hybridization Probes [J].

Ghosh, Sournitra S. ;

Kao, Philip M. ;

McCue, Ann W. ;

Chappelle, Hugh L. .

BIOCONJUGATE CHEMISTRY, 1990, 1 (01) :71-76

[6] THE SOLID-PHASE SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING A 3'-PEPTIDE MOIETY [J].

HARALAMBIDIS, J ;

DUNCAN, L ;

TREGEAR, GW .

TETRAHEDRON LETTERS, 1987, 28 (43) :5199-5202

[7] THE SYNTHESIS OF POLYAMIDE OLIGONUCLEOTIDE CONJUGATE MOLECULES [J].

HARALAMBIDIS, J ;

DUNCAN, L ;

ANGUS, K ;

TREGEAR, GW .

NUCLEIC ACIDS RESEARCH, 1990, 18 (03) :493-499

[8] FACILE PREPARATION OF 3'-OLIGONUCLEOTIDE-PEPTIDE CONJUGATES [J].

JUBY, CD ;

RICHARDSON, CD ;

BROUSSEAU, R .

TETRAHEDRON LETTERS, 1991, 32 (07) :879-882

[9] Biological Activity of Oligonucleotide-Poly(L-lysine) Conjugates: Mechanism of Cell Uptake [J].

Leonetti, Jean-Paul ;

Degols, Genevieve ;

Lebleu, Bernard .

BIOCONJUGATE CHEMISTRY, 1990, 1 (02) :149-153

[10] NICKEL-MEDIATED SEQUENCE-SPECIFIC OXIDATIVE CLEAVAGE OF DNA BY A DESIGNED METALLOPROTEIN [J].

MACK, DP ;

DERVAN, PB .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (11) :4604-4606