Purine Biosynthesis Mutants (purA and purB) of Serotype 4b Listeria monocytogenes Are Severely Attenuated for Systemic Infection in Intragastrically Inoculated A/J Mice

被引：17

作者：

Faith, Nancy G. ^{[1
,2
]}

Kim, Jae-Won ^{[3
]}

Azizoglu, Reha ^{[3
]}

Kathariou, Sophia ^{[3
]}

Czuprynski, Charles ^{[1
,2
]}

机构：

[1] Univ Wisconsin Madison, Sch Vet Med, Madison, WI 53706 USA

[2] Univ Wisconsin Madison, Food Res Inst, Madison, WI 53706 USA

[3] N Carolina State Univ, Dept Food Bioproc & Nutr Sci, Raleigh, NC 27695 USA

来源：

FOODBORNE PATHOGENS AND DISEASE | 2012年 / 9卷 / 05期

关键词：

COMPARATIVE GENOMICS; BACILLUS-ANTHRACIS; VIRULENCE; STRAIN; GROWTH; MOUSE; F2365;

D O I：

10.1089/fpd.2011.1013

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

In this study, we demonstrate that purA and purB transposon mutants of serotype 4b Listeria monocytogenes were severely impaired in their ability to colonize the gastrointestinal tract and cause systemic infection of the spleen, liver, and gallbladder following intragastric inoculation of A/J mice. The mutant strains were also impaired in their ability to multiply within Caco-2 human intestinal epithelial cells. Neither mutant was affected in resistance to synthetic gastric fluid (pH 4.5). These findings indicate that purine biosynthesis is critical for gastrointestinal virulence of L. monocytogenes serotype 4b in mice.

引用

页码：480 / 486

页数：7

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