Production, purification and characterization of reutericin 6, a bacteriocin with lytic activity produced by Lactobacillus reuteri LA6

被引：60

作者：

Kabuki, T ^{[1
]}

Saito, T ^{[1
]}

Kawai, Y ^{[1
]}

Uemura, J ^{[1
]}

Itoh, T ^{[1
]}

机构：

[1] TOHOKU UNIV,FAC AGR,DEPT APPL BIO SCI,LAB ANIM PRODUCTS CHEM,AOBA KU,SENDAI,MIYAGI 981,JAPAN

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 1997年 / 34卷 / 02期

关键词：

Reutericin; 6; bacteriocin; Lactobacillus reuteri; lysis; purification;

D O I：

10.1016/S0168-1605(96)01180-4

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A bacteriocin (Reutericin 6) produced by Lactobacillis reuteri LA6, was purified by hydrophobic chromatography from the modified MRS broth (D'-MRS) with 6180-fold increase in specific activity with 14% recovery. The molecular weight of reutericin 6 was determined to be 2.7 kDa by SDS-PAGE and ESI-MS. By amino acid analysis, reutericin 6 comprised of 67% hydrophobic and polar neutral amino acids. Lanthionine was not detected. The lytic activity against Lactobacillus delbruekii subsp. bulgaricus JCM 1002(T) and NIAI B6 was detected by the decrease of both turbidity and the number of viable cells, and by leaking of beta-galactosidase.

引用

页码：145 / 156

页数：12

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