Molecular cloning of novel Monad binding protein containing tetratricopeptide repeat domains

被引：32

作者：

Itsuki, Yuki ^{[1
,3
]}

Saeki, Makio ^{[1
]}

Nakahara, Hirokazu ^{[2
]}

Egusa, Hiroshi ^{[3
]}

Irie, Yasuyuki ^{[4
]}

Terao, Yutaka ^{[5
]}

Kawabata, Shigetada ^{[5
]}

Yatani, Hirofumi ^{[3
]}

Kamisaki, Yoshinori ^{[1
,6
]}

机构：

[1] Osaka Univ, Grad Sch Dent, Dept Pharmacol, Suita, Osaka 5650871, Japan

[2] Osaka Univ, Dept Oral & Maxillofacial Surg 1, Suita, Osaka 5650871, Japan

[3] Osaka Univ, Grad Sch Dent, Dept Fixed Prosthodont, Suita, Osaka 5650871, Japan

[4] Iwate Med Univ, Dept Pharmacol, Morioka, Iwate 020, Japan

[5] Osaka Univ, Grad Sch Dent, Dept Oral & Mol Microbiol, Suita, Osaka 5650871, Japan

[6] Shanghai Univ E Inst, Div Nitr Oxide & Inflammatory Med, Shanghai, Peoples R China

来源：

FEBS LETTERS | 2008年 / 582卷 / 16期

基金：

日本学术振兴会;

关键词：

apoptosis; cell death; caspase; WD repeat; TPR; TNF-alpha;

D O I：

10.1016/j.febslet.2008.05.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have previously reported that Monad, a novel WD40 repeat protein, potentiates apoptosis induced by tumor necrosis factor-alpha(TNF-alpha) and cycloheximide (CHX). By affinity purification and mass spectrometry, we identified RNA polymerase II-associated protein 3 ( RPAP3) as a binding protein of Monad. Overexpression of RPAP3 in HEK 293 potentiated caspase-3 activation and apoptosis induced by TNF-alpha and CHX. In addition, knockdown of RPAP3 by RNA interference resulted in a significant reduction of apoptosis induced by TNF-alpha and CHX in HEK293 and HeLa cells. These results raise the possibility that RPAP3, together with Monad, may function as a novel modulator of apoptosis pathway.

引用

页码：2365 / 2370

页数：6

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